1. Sacks HS, Fain JN. Human epicardial adipose tissue: a review. Am Heart J.
2007;153(6):907-917.
2. Iacobellis G, Corradi D, Sharma AM. Epicardial adipose tissue: anatomic, biomolecular and clinical relationships with the heart. Nature clinical practice Cardiovascular medicine. 2005;2(10):536-543.
3. Abbara S, Desai JC, Cury RC, Butler J, Nieman K, Reddy V. Mapping epicardial fat with multi-detector computed tomography to facilitate percutaneous transepicardial arrhythmia ablation. European journal of radiology. 2006;57(3):417-422.
4. Mazurek T, Zhang L, Zalewski A, Mannion JD, Diehl JT, Arafat H, Sarov-Blat L, O'Brien S, Keiper EA, Johnson AG, Martin J, Goldstein BJ, Shi Y.
Human epicardial adipose tissue is a source of inflammatory mediators.
Circulation. 2003;108(20):2460-2466.
5. Gollasch M, Dubrovska G. Paracrine role for periadventitial adipose tissue in the regulation of arterial tone. Trends Pharmacol Sci. 2004;25(12):647-653.
6. Ishii T, Asuwa N, Masuda S, Ishikawa Y. The effects of a myocardial bridge on coronary atherosclerosis and ischaemia. J Pathol. 1998;185(1):4-9.
7. Baker AR, Silva NF, Quinn DW, Harte AL, Pagano D, Bonser RS, Kumar S, McTernan PG. Human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular disease. Cardiovascular diabetology. 2006;5:1.
8. Iacobellis G, Pistilli D, Gucciardo M, Leonetti F, Miraldi F, Brancaccio G, Gallo P, di Gioia CR. Adiponectin expression in human epicardial adipose tissue in vivo is lower in patients with coronary artery disease. Cytokine.
2005;29(6):251-255.
9. Ho E, Shimada Y. Formation of the epicardium studied with the scanning electron microscope. Dev Biol. 1978;66(2):579-585.
10. Malavazos AE, Ermetici F, Coman C, Corsi MM, Morricone L, Ambrosi B.
Influence of epicardial adipose tissue and adipocytokine levels on cardiac abnormalities in visceral obesity. International Journal of Cardiology.
2007;121(1):132-134.
11. Sironi AM, Gastaldelli A, Mari A, Ciociaro D, Positano V, Postano V, Buzzigoli E, Ghione S, Turchi S, Lombardi M, Ferrannini E. Visceral fat in hypertension: influence on insulin resistance and beta-cell function.
Hypertension. 2004;44(2):127-133.
12. Iacobellis G, Assael F, Ribaudo MC, Zappaterreno A, Alessi G, Di Mario U, Leonetti F. Epicardial fat from echocardiography: a new method for visceral adipose tissue prediction. Obes Res. 2003;11(2):304-310.
13. Iacobellis G, Leonetti F. Epicardial adipose tissue and insulin resistance in obese subjects. Journal of Clinical Endocrinology & Metabolism.
2005;90(11):6300-6302.
14. Iacobellis G, Ribaudo MC, Zappaterreno A, Iannucci CV, Leonetti F. Relation between epicardial adipose tissue and left ventricular mass. Am J Cardiol.
2004;94(8):1084-1087.
63 15. Iacobellis G, Leonetti F, Singh N, M Sharma A. Relationship of epicardial
adipose tissue with atrial dimensions and diastolic function in morbidly obese subjects. International Journal of Cardiology. 2007;115(2):272-273.
16. Jeong JW, Jeong MH, Yun KH, Oh SK, Park EM, Kim YK, Rhee SJ, Lee EM, Lee J, Yoo NJ, Kim NH, Park JC. Echocardiographic epicardial fat thickness and coronary artery disease. Circ J. 2007;71(4):536-539.
17. Taguchi R, Takasu J, Itani Y, Yamamoto R, Yokoyama K, Watanabe S, Masuda Y. Pericardial fat accumulation in men as a risk factor for coronary artery disease. Atherosclerosis. 2001;157(1):203-209.
18. Chaowalit N, Somers VK, Pellikka PA, Rihal CS, Lopez-Jimenez F.
Subepicardial adipose tissue and the presence and severity of coronary artery disease. Atherosclerosis. 2006;186(2):354-359.
19. Kessels K, Cramer MJ, Velthuis B. Epicardial adipose tissue imaged by magnetic resonance imaging: an important risk marker of cardiovascular disease. Heart. 2006;92(7):962.
20. Greenland P, Bonow RO, Brundage BH, Budoff MJ, Eisenberg MJ, Grundy SM, Lauer MS, Post WS, Raggi P, Redberg RF, Rodgers GP, Shaw LJ, Taylor AJ, Weintraub WS, Tomography) ACoCFCECTFAAWCtUtECDoEBC, Prevention SoAIa, Tomography SoCC. ACCF/AHA 2007 clinical expert consensus document on coronary artery calcium scoring by computed tomography in global cardiovascular risk assessment and in evaluation of patients with chest pain: a report of the American College of Cardiology Foundation Clinical Expert Consensus Task Force (ACCF/AHA Writing Committee to Update the 2000 Expert Consensus Document on Electron Beam Computed Tomography) developed in collaboration with the Society of Atherosclerosis Imaging and Prevention and the Society of Cardiovascular Computed Tomography. J Am Coll Cardiol. 2007;49(3):378-402.
21. O'Rourke RA, Brundage BH, Froelicher VF, Greenland P, Grundy SM, Hachamovitch R, Pohost GM, Shaw LJ, Weintraub WS, Winters WL.
American College of Cardiology/American Heart Association Expert Consensus Document on electron-beam computed tomography for the diagnosis and prognosis of coronary artery disease. Journal of the American College of Cardiology. 2000;36(1):326-340.
22. Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s.
Nature. 1993;362(6423):801-809.
23. Stary HC, Chandler AB, Dinsmore RE, Fuster V, Glagov S, Insull W, Rosenfeld ME, Schwartz CJ, Wagner WD, Wissler RW. A definition of advanced types of atherosclerotic lesions and a histological classification of atherosclerosis. A report from the Committee on Vascular Lesions of the Council on Arteriosclerosis, American Heart Association. Circulation.
1995;92(5):1355-1374.
24. Tanenbaum SR, Kondos GT, Veselik KE, Prendergast MR, Brundage BH, Chomka EV. Detection of calcific deposits in coronary arteries by ultrafast computed tomography and correlation with angiography. Am J Cardiol.
1989;63(12):870-872.
25. Mieres JH, Shaw LJ, Arai A, Budoff MJ, Flamm SD, Hundley WG, Marwick TH, Mosca L, Patel AR, Quinones MA, Redberg RF, Taubert KA, Taylor AJ, Thomas GS, Wenger NK, Cardiac Imaging Committee CoCC, and the Cardiovascular Imaging and Intervention Committee, Council on Cardiovascular Radiology and Intervention, American Heart Association. Role
64 of noninvasive testing in the clinical evaluation of women with suspected coronary artery disease: Consensus statement from the Cardiac Imaging Committee, Council on Clinical Cardiology, and the Cardiovascular Imaging and Intervention Committee, Council on Cardiovascular Radiology and Intervention, American Heart Association. Circulation. 2005;111(5):682-696.
26. Kennedy J, Shavelle R, Wang S, Budoff M, Detrano RC. Coronary calcium and standard risk factors in symptomatic patients referred for coronary angiography. Am Heart J. 1998;135(4):696-702.
27. Beckman JA, Ganz J, Creager MA, Ganz P, Kinlay S. Relationship of clinical presentation and calcification of culprit coronary artery stenoses. Arterioscler Thromb Vasc Biol. 2001;21(10):1618-1622.
28. Falk E, Shah PK, Fuster V. Coronary plaque disruption. Circulation.
1995;92(3):657-671.
29. Davies MJ. The composition of coronary-artery plaques. N Engl J Med.
1997;336(18):1312-1314.
30. Arad Y, Goodman KJ, Roth M, Newstein D, Guerci AD. Coronary calcification, coronary disease risk factors, C-reactive protein, and atherosclerotic cardiovascular disease events: the St. Francis Heart Study.
Journal of the American College of Cardiology. 2005;46(1):158-165.
31. Taylor AJ, Bindeman J, Feuerstein I, Cao F, Brazaitis M, O'Malley PG.
Coronary calcium independently predicts incident premature coronary heart disease over measured cardiovascular risk factors: mean three-year outcomes in the Prospective Army Coronary Calcium (PACC) project. Journal of the American College of Cardiology. 2005;46(5):807-814.
32. Vliegenthart R, Oudkerk M, Hofman A, Oei HH, van Dijck W, van Rooij FJ, Witteman JC. Coronary calcification improves cardiovascular risk prediction in the elderly. Circulation. 2005;112(4):572-577.
33. Burke AP, Taylor A, Farb A, Malcom GT, Virmani R. Coronary calcification:
insights from sudden coronary death victims. Zeitschrift für Kardiologie.
2000;89 Suppl 2:49-53.
34. Burke AP, Weber DK, Kolodgie FD, Farb A, Taylor AJ, Virmani R.
Pathophysiology of calcium deposition in coronary arteries. Herz.
2001;26(4):239-244.
35. Ehara S, Kobayashi Y, Yoshiyama M, Shimada K, Shimada Y, Fukuda D, Nakamura Y, Yamashita H, Yamagishi H, Takeuchi K, Naruko T, Haze K, Becker AE, Yoshikawa J, Ueda M. Spotty calcification typifies the culprit plaque in patients with acute myocardial infarction: an intravascular ultrasound study. Circulation. 2004;110(22):3424-3429.
36. Rumberger JA, Simons DB, Fitzpatrick LA, Sheedy PF, Schwartz RS.
Coronary artery calcium area by electron-beam computed tomography and coronary atherosclerotic plaque area. A histopathologic correlative study.
Circulation. 1995;92(8):2157-2162.
37. Agatston AS, Janowitz WR, Hildner FJ, Zusmer NR, Viamonte M, Detrano R.
Quantification of coronary artery calcium using ultrafast computed tomography. Journal of the American College of Cardiology. 1990;15(4):827-832.
38. Kiernan TJ, Boilson B. Endothelial progenitor cells in 2006--where are we now? Cardiovasc Pathol. 2006;15(4):236-239.
39. Aicher A, Zeiher AM, Dimmeler S. Mobilizing endothelial progenitor cells.
Hypertension. 2005;45(3):321-325.
65 40. Fadini GP, Sartore S, Albiero M, Baesso I, Murphy E, Menegolo M, Grego F,
Vigili de Kreutzenberg S, Tiengo A, Agostini C, Avogaro A. Number and function of endothelial progenitor cells as a marker of severity for diabetic vasculopathy. Arterioscler Thromb Vasc Biol. 2006;26(9):2140-2146.
41. Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, Witzenbichler B, Schatteman G, Isner JM. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997;275(5302):964-967.
42. Vasa M, Fichtlscherer S, Aicher A, Adler K, Urbich C, Martin H, Zeiher AM, Dimmeler S. Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease. Circ Res. 2001;89(1):E1-7.
43. Hill JM, Zalos G, Halcox JP, Schenke WH, Waclawiw MA, Quyyumi AA, Finkel T. Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. N Engl J Med. 2003;348(7):593-600.
44. Oliveras A, Soler MJ, Martínez-Estrada OM, Vázquez S, Marco-Feliu D, Vila JS, Vilaró S, Lloveras J. Endothelial progenitor cells are reduced in refractory hypertension. Journal of human hypertension. 2008;22(3):183-190.
45. Tepper OM, Galiano RD, Capla JM, Kalka C, Gagne PJ, Jacobowitz GR, Levine JP, Gurtner GC. Human endothelial progenitor cells from type II diabetics exhibit impaired proliferation, adhesion, and incorporation into vascular structures. Circulation. 2002;106(22):2781-2786.
46. Loomans CJ, de Koning EJ, Staal FJ, Rookmaaker MB, Verseyden C, de Boer HC, Verhaar MC, Braam B, Rabelink TJ, van Zonneveld AJ. Endothelial progenitor cell dysfunction: a novel concept in the pathogenesis of vascular complications of type 1 diabetes. Diabetes. 2004;53(1):195-199.
47. Takahashi T, Kalka C, Masuda H, Chen D, Silver M, Kearney M, Magner M, Isner JM, Asahara T. Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization. Nat Med.
1999;5(4):434-438.
48. Shintani S, Murohara T, Ikeda H, Ueno T, Honma T, Katoh A, Sasaki K, Shimada T, Oike Y, Imaizumi T. Mobilization of endothelial progenitor cells in patients with acute myocardial infarction. Circulation. 2001;103(23):2776-2779.
49. Gill M, Dias S, Hattori K, Rivera ML, Hicklin D, Witte L, Girardi L, Yurt R, Himel H, Rafii S. Vascular trauma induces rapid but transient mobilization of VEGFR2(+)AC133(+) endothelial precursor cells. Circ Res. 2001;88(2):167-174.
50. George J, Goldstein E, Abashidze S, Deutsch V, Shmilovich H, Finkelstein A, Herz I, Miller H, Keren G. Circulating endothelial progenitor cells in patients with unstable angina: association with systemic inflammation. European Heart Journal. 2004;25(12):1003-1008.
51. Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S. Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand. Cell. 2002;109(5):625-637.
52. Vasa M, Fichtlscherer S, Adler K, Aicher A, Martin H, Zeiher AM, Dimmeler S. Increase in circulating endothelial progenitor cells by statin therapy in patients with stable coronary artery disease. Circulation. 2001;103(24):2885-2890.
66 53. Llevadot J, Murasawa S, Kureishi Y, Uchida S, Masuda H, Kawamoto A,
Walsh K, Isner JM, Asahara T. HMG-CoA reductase inhibitor mobilizes bone marrow--derived endothelial progenitor cells. J Clin Invest. 2001;108(3):399-405.
54. Deschaseaux F, Selmani Z, Falcoz P-E, Mersin N, Meneveau N, Penfornis A, Kleinclauss C, Chocron S, Etievent J-P, Tiberghien P, Kantelip J-P, Davani S.
Two types of circulating endothelial progenitor cells in patients receiving long term therapy by HMG-CoA reductase inhibitors. Eur J Pharmacol.
2007;562(1-2):111-118.
55. Spiel AO, Mayr FB, Leitner JM, Firbas C, Sieghart W, Jilma B. Simvastatin and rosuvastatin mobilize Endothelial Progenitor Cells but do not prevent their acute decrease during systemic inflammation. Thromb Res. 2008;123(1):108-113.
56. Leone AM, Rutella S, Giannico MB, Perfetti M, Zaccone V, Brugaletta S, Garramone B, Niccoli G, Porto I, Liuzzo G, Biasucci LM, Bellesi S, Galiuto L, Leone G, Rebuzzi AG, Crea F. Effect of intensive vs standard statin therapy on endothelial progenitor cells and left ventricular function in patients with acute myocardial infarction: Statins for regeneration after acute myocardial infarction and PCI (STRAP) trial. International Journal of Cardiology.
2008;130(3):457-462.
57. Hristov M, Fach C, Becker C, Heussen N, Liehn EA, Blindt R, Hanrath P, Weber C. Reduced numbers of circulating endothelial progenitor cells in patients with coronary artery disease associated with long-term statin treatment. Atherosclerosis. 2007;192(2):413-420.
58. Imanishi T, Hano T, Nishio I. Angiotensin II accelerates endothelial progenitor cell senescence through induction of oxidative stress. J Hypertens.
2005;23(1):97-104.
59. Imanishi T, Hano T, Nishio I. Estrogen reduces endothelial progenitor cell senescence through augmentation of telomerase activity. J Hypertens.
2005;23(9):1699-1706.
60. Schmidt-Lucke C, Rössig L, Fichtlscherer S, Vasa M, Britten M, Kämper U, Dimmeler S, Zeiher AM. Reduced number of circulating endothelial progenitor cells predicts future cardiovascular events: proof of concept for the clinical importance of endogenous vascular repair. Circulation.
2005;111(22):2981-2987.
61. Chen J, Chopp M. Neurorestorative treatment of stroke: cell and pharmacological approaches. NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics. 2006;3(4):466-473.
62. Povsic TJ, Goldschmidt-Clermont PJ. Endothelial progenitor cells: markers of vascular reparative capacity. Therapeutic advances in cardiovascular disease.
2008;2(3):199-213.
63. Aoki J, Serruys PW, van Beusekom H, Ong AT, McFadden EP, Sianos G, van der Giessen WJ, Regar E, de Feyter PJ, Davis HR, Rowland S, Kutryk MJ.
Endothelial progenitor cell capture by stents coated with antibody against CD34: the HEALING-FIM (Healthy Endothelial Accelerated Lining Inhibits Neointimal Growth-First In Man) Registry. Journal of the American College of Cardiology. 2005;45(10):1574-1579.
64. Miglionico M, Patti G, D'Ambrosio A, Di Sciascio G. Percutaneous coronary intervention utilizing a new endothelial progenitor cells antibody-coated stent:
a prospective single-center registry in high-risk patients. Catheterization and
67 cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions. 2008;71(5):600-604.
65. Co M, Tay E, Lee CH, Poh KK, Low A, Lim J, Lim IH, Lim YT, Tan HC.
Use of endothelial progenitor cell capture stent (Genous Bio-Engineered R Stent) during primary percutaneous coronary intervention in acute myocardial infarction: intermediate- to long-term clinical follow-up. Am Heart J.
2008;155(1):128-132.
66. Werner N, Kosiol S, Schiegl T, Ahlers P, Walenta K, Link A, Böhm M, Nickenig G. Circulating endothelial progenitor cells and cardiovascular outcomes. N Engl J Med. 2005;353(10):999-1007.
67. Moreno PR, Sanz J, Fuster V. Promoting mechanisms of vascular health:
circulating progenitor cells, angiogenesis, and reverse cholesterol transport.
Journal of the American College of Cardiology. 2009;53(25):2315-2323.
68. Shantsila E, Watson T, Lip GYH. Endothelial progenitor cells in cardiovascular disorders. J Am Coll Cardiol. 2007;49(7):741-752.
69. Wang HY, Gao PJ, Ji KD, Shen WF, Fan CL, Lu L, Zhu DL. Circulating endothelial progenitor cells, C-reactive protein and severity of coronary stenosis in Chinese patients with coronary artery disease. Hypertens Res.
2007;30(2):133-141.
70. Müller-Ehmsen J, Braun D, Schneider T, Pfister R, Worm N, Wielckens K, Scheid C, Frommolt P, Flesch M. Decreased number of circulating progenitor cells in obesity: beneficial effects of weight reduction. European Heart Journal. 2008;29(12):1560-1568.
71. Hughes AD, Coady E, Raynor S, Mayet J, Wright AR, Shore AC, Kooner JS, Thom SA, Chaturvedi N. Reduced endothelial progenitor cells in European and South Asian men with atherosclerosis. Eur J Clin Invest. 2007;37(1):35-41.
72. Fadini GP, Coracina A, Baesso I, Agostini C, Tiengo A, Avogaro A, de Kreutzenberg SV. Peripheral blood CD34+KDR+ endothelial progenitor cells are determinants of subclinical atherosclerosis in a middle-aged general population. Stroke. 2006;37(9):2277-2282.
73. Chironi G, Walch L, Pernollet M, Gariepy J, Levenson J, Rendu F, Simon A.
Decreased number of circulating CD34+KDR+ cells in asymptomatic subjects with preclinical atherosclerosis. Atherosclerosis. 2007;191(1):115-120.
74. Snell-Bergeon JK, Hokanson JE, Kinney GL, Dabelea D, Ehrlich J, Eckel RH, Ogden L, Rewers M. Measurement of abdominal fat by CT compared to waist circumference and BMI in explaining the presence of coronary calcium. Int J Obes Relat Metab Disord. 2004;28(12):1594-1599.
75. See R, Abdullah SM, McGuire DK, Khera A, Patel MJ, Lindsey JB, Grundy SM, de Lemos JA. The association of differing measures of overweight and obesity with prevalent atherosclerosis: the Dallas Heart Study. J Am Coll Cardiol. 2007;50(8):752-759.
76. Jung C, Fischer N, Fritzenwanger M, Thude H, Ferrari M, Fabris M, Brehm BR, Barz D, Figulla HR. Endothelial progenitor cells in adolescents: impact of overweight, age, smoking, sport and cytokines in younger age. Clinical research in cardiology : official journal of the German Cardiac Society.
2009;98(3):179-188.
77. Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in
68 Adults (Adult Treatment Panel III) Final Report. Circulation.
2002;106(25):3143-.
78. Clinical guidelines on the identification, evaluation, and treatment of overweight and obesity in adults: executive summary. Expert Panel on the Identification, Evaluation, and Treatment of Overweight in Adults. Am J Clin Nutr. 1998;68(4):899-917.
79. Borkan GA, Gerzof SG, Robbins AH, Hults DE, Silbert CK, Silbert JE.
Assessment of abdominal fat content by computed tomography. Am J Clin Nutr. 1982;36(1):172-177.
80. Yoshizumi T, Nakamura T, Yamane M, Islam AH, Menju M, Yamasaki K, Arai T, Kotani K, Funahashi T, Yamashita S, Matsuzawa Y. Abdominal fat:
standardized technique for measurement at CT. Radiology. 1999;211(1):283-286.
81. Ashwell M, Cole TJ, Dixon AK. Obesity: new insight into the anthropometric classification of fat distribution shown by computed tomography. British medical journal (Clinical research ed). 1985;290(6483):1692-1694.
82. Sanz J DS, Fuster V, Rajagopalan S. Calcium scoring and contrast-enhanced CT angiography. Curr Mol Med. 2006;6(5):525-539.
83. Bettencourt N, Rocha J, Carvalho M, Leite D, Toschke A, Melica B, Santos L, Rodrigues A, Goncalves M, Braga P, Teixeira M, Simoes L, Rajagopalan S, Gama V. Multislice Computed Tomography in the Exclusion of Coronary Artery Disease in Patients With Presurgical Valve Disease. Circulation:
Cardiovascular Imaging. 2009;2(4):306-313.
84. Austen WG, Edwards JE, Frye RL, Gensini GG, Gott VL, Griffith LS, McGoon DC, Murphy ML, Roe BB. A reporting system on patients evaluated for coronary artery disease. Report of the Ad Hoc Committee for Grading of Coronary Artery Disease, Council on Cardiovascular Surgery, American Heart Association. Circulation. 1975;51(4 Suppl):5-40.
85. Kondo T, Hayashi M, Takeshita K, Numaguchi Y, Kobayashi K, Iino S, Inden Y, Murohara T. Smoking cessation rapidly increases circulating progenitor cells in peripheral blood in chronic smokers. Arterioscler Thromb Vasc Biol.
2004;24(8):1442-1447.
86. Lambiase PD, Edwards RJ, Anthopoulos P, Rahman S, Meng YG, Bucknall CA, Redwood SR, Pearson JD, Marber MS. Circulating humoral factors and endothelial progenitor cells in patients with differing coronary collateral support. Circulation. 2004;109(24):2986-2992.
87. Fadini GP, Agostini C, Avogaro A. Characterization of endothelial progenitor cells. Biochem Biophys Res Commun. 2005;336(1):1-2.
88. Bongartz G, Golding SJ, Jurik AG, Leonardi M, Meerten EvPv, Rodríguez R, Schneider K, Calzado A, Geleijns J, Jessen K, Panzer W, Shrimpton PC, Tosi G. European Guidelines for Multislice Computed Tomography. Funded by the European Commission. Contract number FIGM-CT2000-20078-CT-TIP.
March 2004.
89. Commission E. European Guidelines on quality criteria for computer tomography. EUR 16262 EN. Luxembourg: Office for Official Publication of the European Communities; 2000.
90. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986;1(8476):307-310.