Com base nos resultados obtidos neste estudo, pode-se concluir que:
1. Os TAMs são capazes de secretar exossomos quando diferenciados em monócitos a partir de células THP1 e quando polarizados em macrófagos M1 e M2. Este achado foi confirmado pela presença de exossomos positivos para o marcador de superfície CD63;
2. Os exossomos derivados de TAMs M1 e das células THP1 apresentaram escassa marcação para TGF- . Não obstante os exossomos derivados dos macrófagos M2 apresentarem positividade para TGF- . No que concerne ao exossomos derivados das linhagens celulares SCC-25, SAS e HSC-3, não foi possível observar a presença de marcação para esta proteína, sugerindo que os exossomos das células de CE de língua não apresentam TGF- em seu interior;
3. As células SCC-25 cultivadas juntamente com os exossomos, no ensaio de invasão utilizando o meio de cultura DMEM F/12, apresentaram maior tendência de invasão através do miogel para a concentração 10 µg/ml dos exossomos derivados de M2. A linhagem celular HSC-3 apresentou menor capacidade invasiva em meio DMEM F/12. No meio de cultura com ausência de FBS, percebeu-se que houve uma maior capacidade invasiva pelas células HSC-3, de forma quantitativa, apesar da ausência de relação estatisticamente significativa. A linhagem de células SAS não apresentou maior capacidade invasiva quando comparados os diferentes meios de cultura;
4. Quanto à capacidade de atração das linhagens celulares pelos exossomos, foi possível inferir que a linhagem celular SCC-25 não apresentou maior capacidade de invasão quando os exossomos estavam localizados no compartimento inferior do transwell. Ao passo que a linhagem HSC-3 apresentou maior capacidade de invadir quando em presença dos exossomos M2 (1,0 µg/ml) no compartimento inferior do transwell;
5. Os ensaios de proliferação e viabilidade celular utilizando a linhagem HSC-3 apresentaram discreto aumento na viabilidade celular em presença de exossomos M2 (1,0 µg/ml) em meio de cultura DMEM F/12. A linhagem celular SCC-25 apresentou aumento na proliferação em presença de exossomos M2 (50 µg/ml) para ambos os meios de culturas estudados;
6. O estudo da imunofluorescência possibilitou a observação de que exossomos derivados de TAMs apresentam capacidade de internalização nas células de CE de língua.
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