A fonte de luz de 1,37 GeV de segunda geração, com suas primeiras sete linhas de luz, foi inaugurada em 1997. Atualmente, as 16 linhas de luz em operação no LNLS atendem anualmente cerca de 2.300 usuários que realizam mais de 450 projetos de pesquisa. Para criar um canal de comunicação direto entre os usuários, os cientistas da linha de luz e a direção do Laboratório criaram um Comitê em 2010.
Biologia Estrutural Sessão 1 Sala Amadeus
Chairperson: Rodrigo Villares Portugal
Propriedades Estruturais, Eletrônicas e Magnéticas de Sólidos Sessão 2 Sala 2
Chairperson: Valmor Roberto Mastelaro
Superfícies, Interfaces e Nanossistemas Sessão 3 Sala 5
Chairperson: Maria Luiza Miranda Rocco
Ciência Atômica Sessão 4 Sala 8
Chairperson: Danilo de Paiva Almeida
Materiais Estruturais e Aplicações na Indústria Sessão 5 Sala 9
Chairperson: Álvaro Saavedra
Geociência, Meio-ambiente e Aplicações em Materiais Biológicos Sessão 1 Sala Amadeus
Chairperson: Simone Coutinho Cardoso
Matéria Mole e Fluídos Complexos Sessão 2 Sala 2
Superfícies, Interfaces e Nanossistemas Sessão 3 Sala 5
Chairperson: Richard Landers
Biologia Estrutural Sessão 4 Sala 8
Chairperson: Ricardo Aparicio
Propriedades Estruturais, Eletrônicas e Magnéticas de Sólidos Sessão 5 Sala 9
Chairperson: Fábio Furlan Ferreira
Sessão Temática I: Linhas de Luz XRD / XPD
Chairperson: Guinther Kellermann
Sessão Temática I: Linhas de Luz TGM / SGM / PGM
Chairperson: Maria Luiza Rocco Duarte Pereira 14:00-14:15 Status e perspectivas da linha TGM
Sessão Temática I: Linhas de Luz MX1 / MX2
Chairperson: Humberto D'Muniz Pereira
Sessão Temática II: Linhas de Luz XAFS / DXAS
Chairperson: Félix G. Requejo
Sessão Temática II: Linhas de Luz XRF
Chairperson: Guillermina Bongiovanni
Sessão Temática II: Linhas de Luz SAXS1 / SAXS2
Chairperson: Aldo Craievich
Instrumentation for SAXS studies of surfaces: Rafael G. Oliveira
Sessão Temática II: Linhas de Luz SXS
Chairperson: Jonder Morais
Discussão sobre eventuais necessidades de novas instrumentações I Apreciação crítica sobre aspectos técnicos do funcionamento atual
Comentários sobre o atendimento técnico e administrativo recebido pelos usuários durante a realização de suas experiências no
Sugestões referentes à formação e treinamento dos usuários
150 Study of the influence of reducing agents in the Eu reduction in the BaAl2O4 host. The biosynthesis of the 21st amino acid, Selenocysteine (Sec U), requires complex enzymatic machinery composed in eubacteria of: Selenocysteine Synthase (SELA), Selenocysteine Specific Elongation Factor (SELB), Selenophosphate Synthetase (SELD) and a specific Selenocysteine Synthase (SELA), Selenocysteine Specific Elongation Factor (SELB), Selenophosphate Synthetase (SELD) and a specific Selenocysteine Synthase (SELA) ). Humberto Pereira D'Muniz for the interpretation of the structural and help of other works.
BASES ESTRUTURAIS DO TRANSPORTE NUCLEAR DAS PROTE´INAS KU70 E KU80 MEDIADAS PELA
A lei de inchaço ideal relacionando a fração volumétrica da membrana (φ m) à periodicidade lamelar (D) é dada por φ m = δ m/D, onde δ m é a espessura da membrana, quando as interações estéricas dominam as interações eletrostáticas e de van der Waals, desvios deste comportamento são esperados [3]. Avaliação de alterações estruturais na PLA2 induzidas por cimentos dentários por espalhamento de raios X em baixo ângulo. Nesse sentido, procuramos avaliar o efeito das modificações estruturais causadas pelo agregado trióxido mineral (MTA), principal componente dos cimentos odontológicos, na estrutura da fosfolipase A2 de Bothrops pirajai (proteína modelo).
In this paper we show for the first time the expression, purification, functional and structural analyzes of X. In this paper we aim at the production, purification and crystallization of the AOX protein from M. Cryoglobulins are immunoglobulin (Ig) type proteins that play a role in important in infections, autoimmune diseases and neoplastic disorders[1].
ANALYSIS OF MEMBRANE PROTEOME AND
SECRETOME IN CELLS OVER-EXPRESSING ADAM17 USING QUANTITATIVE PROTEOMICS
This project aimed to identify secreted proteins by pathogenic Leptospira interrogans serovar Kennewicki strain Pomona Fromm (LPF) through proteomic analyses. Liquid chromatography-based separation followed by automated tandem mass spectrometry was also used to identify secreted proteins. A major obstacle to secretome studies is the difficulty in obtaining extracts of secreted proteins without citoplasmic contamination.
To our knowledge, this is the first study to identify secreted proteins by leptospires using a proteomic approach. The identification of these proteins will contribute to the elucidation of the pathogenic mechanisms and the development of new strategies for the treatment and prevention of leptospirosis. We performed the cloning and overexpression of the cytoplasmic tail of ADAM17 in the HEK-293 cell line and the ligands were determined by LC-MS/MS after immunoprecipitation (IP) of proteins with anti-FLAG M2 Affinity Gel (Sigma).
Trx-1 is part of the peroxiredoxin/thioredoxin/thioredoxin reductase system, one of the mechanisms by which cells maintain a reduced cellular environment and inactivate reactive oxygen species (ROS). These GT-pases are anchored to the surface of the lysosome through interaction with a complex of three proteins, p18, MP1 and p14, called the Ragulator. The aim of this project is to study the interaction between p18 and other components of the Ragulator complex.
Estudos demonstraram que a proteína GIP-1 (Glutaminase Interacting Protein)/TIP-1 (Tax interacting protein 1) é a parceira de interação da enzima LGA, cuja interação foi confirmada em células nervosas caninas.
MOLECULAR MASS DISTRIBUTION AND EPITOPES OF THE BETA LACTOGLOBULIN SUBMITTED TO
TREATMENT
Functional and Biophysical studies on four Ceratoplatanins from the fungus Moniliophthora perniciosa, causative agent of the Witch's Broom Disease. Ceratoplatanin (CP) is a secreted protein of 12.4 kDa that was initially identified in culture filtrates of the ascomycete Ceratocystis fimbriata f. Ion photodesorption experiments on the icy surface were performed at the Brazilian Synchrotron Light Source (LNLS), using the Spherical Grating Monochromator (SGM) beamline, operated in the single group mode of the storage ring, with a period of 311 ns and cluster width of 60ps.
This study examines, in the laboratory, the stability of glycine against the action of ultraviolet radiation, in the spectral region around the wavelength of the Lyman alline (1216) produced by a hydrogen lamp. Using this phenomenon, different depths of a sample surface can be studied by means of the correct choice of the incident radiation angle. Currently, further investigations are being carried out to achieve a complete understanding of the processes involved with the aim of transforming this practical method into a true and complete analytical tool.
Investigation of the ionic fragmentation of dimethyl diselenide, CH3SeSeCH3, with synchrotron radiation around the Se 3p region. In this work, we present a study of the dissociative photoionization of the volatile selenium compound CH3SeSeCH3 using multi-coincidence time-of-flight mass spectrometry and synchrotron radiation in the Se 3p edge. A depth scan of a multilayer sample in confocal setup was performed in the XRF Beamline of LNLS (Campinas, Brazil) [5].
The result is relevant as it allows discrimination of the oxidation state of the elements present in a sample in a 3D micrometer regime using resonant Raman scattering combined with a confocal setup.
SEÇÃO DE MEDIDAS DE CHOQUE ABSOLUTO DE FOTOABSORÇÃO DE MOLÉCULAS POLIATÔMICAS:.
ISOPRENO, ACETONITRILA E HALOTANO
The authors acknowledge the help of the staff of LNLS and the financial support of the Brazilian agencies CNPq and FAPESP. For a better understanding of experimental results, we have made a theoretical analysis of the photofragmentation using Density Functional Theory. The sample was prepared by fluorination of ClSO2NCO using SbF3 and subsequent trap-to-trap distillation in a vacuum line.1 The purity of the compound was carefully checked by infrared spectroscopy.
Most of these studies have focused on the total photoionization cross section, that is, the ionization probability as a function of photon energy. Since the momentum of the system should be conserved in photoionization events, indirect information about the electrons can be obtained by measuring the ions. The measurements were performed at the D09B-XRF beamline XRF station at the Brazilian Synchrotron Facility (LNLS, Campinas).
Nevertheless, this behavior in the residues of compounds could be used to identify the oxidation state of the investigated elements, providing an opportunity for determination of the chemical environment using RRS spectroscopy. The determination of calcium distribution was performed using synchrotron radiation microfluorescence (SR-μXRF) at the X-ray fluorescence beamline of the Brazilian Synchrotron Light Laboratory (LNLS). The formation of the assemblies was confirmed using FT-IR by monitoring the appearance of the carboxylate bands.
The structural characterization by small-angle X-ray scattering (SAXS) made it possible to understand the changes on the shape and size of the aggregates with increasing the concentration of complex salt. From the position of the Bragg peaks, the lamellar periodicity (the thickness of the membranes plus their distance of separation) was determined. Using theoretical model for the shape and structure factors, we fitted that experimental data and determined the thickness of the membranes.
CONTROLLING SILICATE MESO-STRUCTURES
USING SUCUPIRA OIL AS A NEW SWELLING AGENT
As amostras foram analisadas utilizando técnicas de difração de raios X (DRX) e espectroscopia de absorção de raios X (XAS). O espectro de absorção de raios X foi realizado utilizando a linha XAFS-2 (X-ray Absorbing Fine Structure) do Laboratório Nacional de Luz S'íncrotron, Campinas, Brasil (LNLS). Os resultados da espectroscopia de absorção de raios X mostram absorção na borda L3 de Dy.
Agradecimentos: Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM), Universidade do Estado do Amazonas (UEA) e Universidade Estadual de Campinas (UNICAMP). A presença de MMT afeta o índice de inchamento, indicando alteração na capacidade de absorção de água. Esta propriedade pode ser explorada para adaptar a dissolução e liberação de fármacos incorporados nesta matriz.
Os catalisadores de cobre (5, 10 e 20% em massa) foram impregnados em óxido de alumínio (Al) com estrutura hierárquica de poros obtida pelo método sol-gel [1] e caracterizados por fisissorção de N, porosimetria de mercúrio, DRS, XRD e EXAFS. O poliestireno enxertado também desloca o pico de longo período para a região do ângulo inferior, 25 nm–1. Investigação da ativação de catalisadores de Ni ou Ni-Co em alumina modificada com Zn por XANES in situ.
A produção de hidrogênio foi estudada em diferentes temperaturas, entre 200-500oC, sob etanol e vapor de água.
ESTRUTURAS GEOM´ ETRICAS COMPLEXAS
Some researchers have tried to solve this problem - NHS crystals are doped with different types of ionic complexes. The presence of dopants in a crystal lattice can modify the physical properties of these materials for technological applications. High-resolution Renninger scans of NSH crystals were performed at the XRD1 station of the Brazilian Synchrotron Radiation Facility (LNLS). The results show that the symmetries of the crystals are almost the same regardless of the inclusion of manganese ions.
Accurate calculation of the unit cell parameters shows that there is relative dilation of the lattice parameters after Mn doping. The results also showed that reflection phases are sensitive to the presence of dopant ions in the lattice of NSH. Variations in the peak profiles were observed from pure to doped samples, and these variations correlated with shifts in the structure factor phases, triplet phases.
Metal organic frameworks (MOFs) are a large family of materials formed by an inorganic cluster and an organic ligand. These properties can be tailored by choosing different combinations of the metal cluster and the organic ligand, with the aim of specific applications in the fields of gas storage, gas separation, etc. Some MOFs exhibit the property of breathing, which consists of distinct structural changes accompanying the adsorption/desorption of certain molecular species.
Therefore, a technique where both adsorption and structural properties can be assessed at the same time is crucial for their characterization. In this work, we show several examples of in-situ MOFs x-ray diffraction studies performed in controlled environment of gas pressure and temperature. Some of the studied MOFs show the breathing property, with the characteristic structural changes during adsorption/desorption of molecular species in their pores.
A systematic study of the growth of epitaxial graphene on the C face of 4H-SiC at 1775C in Ar atmosphere as a function of time was performed. Raman spectroscopy and X-ray diffraction were measured to study the evolution of structure as a function of growth time. The Raman signal from the multilayer graphene starts to appear after 30 minutes of growth and increases with time.
