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APPL proteins FRET at the BAR: direct observation of APPL1 and APPL2 BAR domain-mediated interactions on cell membranes using FRET microscopy.

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Academic year: 2017

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Figure 1. Summary of FRET donors and acceptors. (A) Immunoblot analysis using an antibody that recognizes both CFP and YFP to show appropriate expression and predicted molecular weight for all six FRET donor and acceptor fusion proteins used in these studi
Figure 2. Flow chart of the confocal microscopy approach for FRET studies. The same cell was used in each of the following steps
Figure 4. Cell images from standard acceptor photobleaching experiments. Shown are representative cells corresponding to two control FRET pairs: (A) CFP + BAR1-YFP and (B) CFP + BAR2-YFP, and three experimental FRET pairs: (C) CFP-BAR1 + BAR1-YFP, (D) CFP-
Figure 6. Emission spectra data for FRET pairs. Shown are emission spectra data from representative cells co-expressing control FRET pairs: (A) CFP + BAR1-YFP and (B) CFP + BAR2-YFP, or three experimental FRET pairs: (C) CFP-BAR1 + BAR1-YFP, (D) CFP-BAR1 +

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