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454 pyrosequencing to describe microbial eukaryotic community composition, diversity and relative abundance: a test for marine haptophytes.

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Academic year: 2017

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Figure 1. Proportional species abundance in 454 pyrosequencing reads from a mock community of haptophytes
Figure 5 shows cladograms of unrooted Neighbor-Joining trees based on unique OTU 99% (including singleton OTUs) from sample DNA-Hap454 and the 11 reference sequences after Initial Filtering (5A), Quality Score (5B), PyroNoise (5C) and  Amplicon-Noise (5D)
Table 4. Comparison of proportional species distribution between the samples and the species distribution in the mock community in terms of cell number and biomass.
Figure S1 Agarose gel loaded with PCR products for clone libraries. From left to right: Ladder (O’GeneRuler 100 bp Plus, Fermentas), template cDNA-bb (from RNA extracted without beater), cDNA+bb (from RNA extracted with  bead-beater), DNA-bb (DNA extracted

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