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Gateway vectors for efficient artificial gene assembly in vitro and expression in yeast Saccharomyces cerevisiae.

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Academic year: 2017

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Table 2. Entry clones created in this study.
Fig. 7A shows a schematic diagram of the gene regulation by AIDtTA in this strain. Without the inducers DOX or NAA, Venus (YFP) fluorescence was clearly visible in the nucleus (Fig
Table 3. Expression plasmid vectors constructed by Gateway recombination method in this study.
Figure 8. Gene expression control by AIDtTA and AIDrtTA: flow cytometry analysis. (A–F) The yeast strains YS129 (-OsTIR1) and YS114 ( + OsTIR1) were transformed with a reporter plasmid construct (TetO 7 -mCherry-NLS) together with the plasmid harboring the

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