Análise das relações filogenéticas entre os gêneros de Cheirodontinae (Ostariophysi: Characiformes: Charasidae) utilizando sequências de DNA mitocondrial e nuclear

ANÁLISE DAS RELAÇÕES FILOGENÉTICAS ENTRE OS

GÊNEROS DE CHEIRODONTINAE (OSTARIOPHYSI:

CHARACIFORMES: CHARACIDAE) UTILIZANDO

SEQUÊNCIAS DE DNA MITOCONDRIAL E NUCLEAR

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FICHA CATALOGRÁFICA ELABORADA PELA SEÇÃO TÉCNICA DE AQUISIÇÃO E TRATAMENTO

DA INFORMAÇÃO

DIVISÃO TÉCNICA DE BIBLIOTECA E DOCUMENTAÇÃO - CAMPUS DE BOTUCATU - UNESP

BIBLIOTECÁRIA RESPONSÁVEL: SELMA MARIA DE JESUS

Mariguela, Tatiane Casagrande.

Análise das relações filogenéticas entre os gêneros de Cheirodontinae

(Ostariophysi: Characiformes: Characidae) utilizando seqüências de DNA

mitocondrial e nuclear / Tatiane Casagrande Mariguela. – Botucatu : [s.n.],

2009.

Tese (doutorado) – Universidade Estadual Paulista, Instituto de Biociências

de Botucatu, 2009

Orientador: Cláudio de Oliveira

Assunto CAPES: 20204000

1. Peixe - Genética 2. DNA 3. Filogenética

CDD 595.55

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Espécie Localidade

Coordenadas

Número

Voucher

Acinocheirodon cf melanogramma

Rio Jequitinhonha/MG

S 16°30'35.0' W 41°20'02.0''

37550

LBP8112

Aphyocheirodon hemigrammus

Rio Paraná/SP

S 22°22'42.4' W 47°25'37.9''

40025

LBP8306

Cheirodon ibicuhiensis

Rio Guaíba/RS

S 30°18'03.9'' W 51°20'40.8''

25598

LBP4777

Cheirodon interruptus

Arroio dos Corrientes/RS

S 31°28'46.3'' W 52°12'46.9''

21266

LBP3381

Cheirodon interruptus

Rio Grande/RS

S 32°09’06.9’’ W 52°06’24.2’’

20486

LBP3343

Cheirodon australe

Rio Quinchilca/Chile

S 39°51'36'’ W 72°45'16''

24979

LBP4732

Cheirodon australe

Lago Pumquipulli/Chile

S 39°36'30'’ W 72°14'01''

24963

LBP4728

Cheirodon killiani

Rio la Laja/Chile

S 37°12'54.8'' W 72°26'49.1''

19803

LBP3115

Cheirodon killiani

Rio Renuico/Chile

S 37°39'16'’ W 72°39'45''

24969

LBP4730

Cheirodon killiani

Rio Duqueco/Chile

S 37°31’27’’ W 72°14’13’’

24974

LBP4731

Cheirodon killiani

Rio Quinchilca/Chile

S 39°51’36’’ W 72°45’16’’

24964

LBP4729

Cheirodon stenodon

Rio Tietê/SP

S 22°45’48.2’’ W 48°15’41.8’’

20130

LBP3015

Compsura gorgonae

Rio Yape/Panamá

N 8,1125 W -77,5914

1

-

Compsura heterura

Natal/RN

S 05°37’47’’ W 35°37’09’’

24984

LBP4733

Compsura sp. nova

Rio Tabasará/Panamá

N 8,2039 W -81,5861

4

-

Genêro e espécie nova

Barra do Garças/MT

S 15°40.678’ W 52°17.863’’

27603

LBP5699

Heterocheirodon yatai

Durazno/Uruguai

S 33°23’49’’ W 56°24’10’’

24954

LBP4872

Kolpotocheirodon figueiredoi

Rio Paraguaçu/BA

S 12°22'19.0'' W 41°31'06.3''

37575

LBP7178

Kolpotocheirodon theloura

Rio Paraná/DF

S 15°43’42.7’ W 47°54’39.4’’

25982

LBP5033

Macropsobrycon uruguayanae

Cachoeira do Sul/RS

S30º14’46’’ W52º45’53’’

29061

LBP6039

Macropsobrycon xinguensis

Rio Tapajós/AM

N 10°12’54,0’’ W 54°58’04,0’’

40499

LBP8731

Nanocheirodon insignis

Venezuela

N 10°09’42.0’ W 72°25’58.0’’

27476

LBP6104

Odontostilbe dialeptura

Rio Santa Maria/Panamá

N 8,1962 W -80,7657

2

-

Odontostilbe ecuadorensis

Santa Cruz/ Bolívia

S 17°34’42” W 63°44’16”

AMNH

102077

Odontostilbe fugitiva

Mâncio Lima/AC

S 07°26’35.5’ W 73°03’33.5’’

23714

LBP4129

Odontostilbe fugitiva

Cruzeiro do Sul/AC

S 7°37’20.0’’ W 72°47’42.2’’

22932

LBP4052

Odontostilbe mitoptera

Rio San Juan Coclesito/Panamá

N 8°80’42” W 80°58’08”

3

-

Odontostilbe paraguayensis

Botucatu/Capivara/SP

S 22°44’ W 48°23’

20129

LBP3486

Odontostilbe pequira

Botucatu/Capivara/SP

S 22°44’ W 48°23’

20124

LBP3013

Odontostilbe pequira

Paysandu/Uruguai

S 32°18’36’’ W 58°05’54’’

24958

LBP4726

Odontostilbe pequira

Coxim/MS

S 18°25’42.5’’ W 54°50’02.8’’

12659

LBP1469

Odontostilbe pulchra

Venezuela

N 07°38’11.6’’ W 66°19’04.2’’

25845

LBP3070

Odontostilbe pulchra

Rio Turure/Panamá

N 10,5897 W -61,0903

8

-

Odontostilbe sp.1

Rio Araquá/SP

S 22°47.135’ W 48°28.892

22626

LBP4650

Odontostilbe sp.2

Rio Grande/SP

S 20°56’49.5’’ W 48°08’51.9’’

29616

LBP6280

Prodontocharax melanotus

Santa Cruz/Bolívia

S 17°41’29” W 63°38’96”

AMNH

102082

Prodontocharax sp.

Rio Amazonas

S -11,8025 W -71,4581

7

-

Pseudocheirodon arnoldi

Rio Iglesias/Panamá

N 8,4231 W -78,0014

5

-

Pseudocheirodon terrabae

Rio Salama Nuevo/Costa Rica

N 8,9043 W -83,4393

6

-

Saccoderma melanostigma

Venezuela

N 10°09’42.0’ W 72°25’58.0’’

27475

LBP6103

Serrapinnus calliurus

Aquidauana/MS

S 19°34’02.3’ W 56°14’09.1’’

25768

LBP3800

Serrapinnus calliurus

Aquidauana/MS

S 19°34’54.6’ W 56°15’16.5’’

22121

LBP3776

Serrapinnus heterodon

Rio Matipó/MG

S 20°18'09.3'' W 42°20'04.6''

20305

LBP3441

Serrapinnus heterodon

Rio São Francisco/MG

S 17°08'54.9' W 43°49'32.3''

37551

LBP8104

Serrapinnus kriegi

Aquidauana/MS

S 19°34’17.3’ W 56°14’44.8’’

25764

LBP4738

Serrapinnus microdon

Rio Purus/AM

S 08°51'21.5'' W 68°42'22.6''

17057

LBP2525

Serrapinnus notomelas

Bauru- Córrego Campo Novo/SP

S 22°20’27.1’’ W 48°56’05.0’’

19890

LBP3861

-&4'1-174<&()(7

25

-540-

301 @&% 9 & % @ 5

C% /

.

Espécie Localidade

Coordenadas

Número

Voucher

Agoniates halecinus

Laranjal do Jari/AP

S 00°34’03’’ W 52°34’41’’

26594

LBP5503

Aphyocharax anisitsi

Coxim/MS

S 18°25'42.5'' W 54°50'02.8''

12660

LBP1470

Aphyocharacidium sp

Rio Negro/AM

N 00°05.610' W 66°49.034''

33167

LBP6875

Astyanax mexicanus

Aquário -

24599

LBP4527

Brycon insignis

Rio Paraíba do Sul/SP

S 22°00’ W 41°20’

16075

LBP2369

Brycon petrosus

Santa Rita Arriba/Panamá

N 09°19’26.2’’ W 79°46’08.2

18511

LBP2750

Bryconops affinis

Represa de Três Marias

S 18°13,661’ W 45°14,857’

4168

LBP262

Carlana eigenmanni

Rio Pizote/Costa Rica

-

19864

LBP3299

Chalceus epakros

Rio Amazonas/PA

S 00°38’46’’ W 52°30’33’’

26504

LBP5443

Charax leticiae

Rio Paraguay/MS

S 18°25’42.5’’ W 54°50’02.8’’

12700

LBP1480

Clupeacharax sp.

Cáceres/MT

S 16°06’56’ W 57°44’33’

26012

LBP5046

Cynopotamus magdalenae

Lago Maracaibo/Venezuela

N 09°38'53.8' W 72°34'56.4''

29515

LBP6132

Diapoma sp

Arroio dos Corrientes/RS

S 31°28'46.3'' W 52°12'46.9''

21274

LBP3383

Glandulocauda melanogenys

Riacho Grande/SP

S 23°46’13.2’’ W 46°18’39.6’’

24538

LBP4507

Gnatocharax sp

Rio Negro/AM

S 00°53'18.6'' W 62°40'36/1''

24494

LBP4496

Hemibrycon sp

Trinidad Tobago

N 10°41.320' W 61°19.499'

33168

LBP6847

Iguanodectes sp.

Igarapé Boiboi/AM

S 00°49’43.7’’ W 62°49’59.8’’

23840

LBP4266

Phenacogaster sp

Rio Paraníba/PI

S 09°09’51’ W 45°51’15’

27299

LBP5582

Piaractus mesopotamicus

Caunesp/SP -

23803

LBP4255

Pseudocorynopoma heterandria

Rio Fau/SP

S 24°12,441’ W 47°28,616’

18570

LBP2862

Stetaprion crenatum

Mâncio Lima/AC

S 07°34'28.8' W 72°55'24.9''

22994

LBP4078

Tetragonopterus argenteus

Rio Negro/MT

S 19°34'33.7' W 56°14'49.5''

22029

LBP3758

<&()(7

GCBD! H

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GCC

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Gene

Identificação dos

Primers

Sequência dos

primers

Referência

Cyt b

L14841

5’- AAA TCA AAG CAT AAC ACT GAA GAT G –3’

Kocher

et al

(1989)

H15915

5’- CCA ATT TGC ATG GAT GTC TTC TCG G –3’

Irwing

et al

(1991)

Cytb08R2

5'- GCT TTG GGA GTT AGD GGT GGG AGT TAG AAT

_{C -3'}

Presente estudo

Cytb -Novo

5'- GAC TTG AAA AAC CAY CGT TGT -3'

Presente estudo

16S rRNA

16Sa-L

5’- ACG CCT GTT TAT CAA AAA CAT – 3’

Palumbi (1996)

16Sb-H

5’- CCG GTC TGA ACT CAG ATC ACG T - 3’

Sia

T3b

5’- ATT AAC CCT CAC TAA AGT CGA GTG CCC CGT

_{GTG YTT YGA YTA- 3’}

Friesen et al. (1999)

T7b

5’- AAT ACG ACT CAC TAT AGG AAG TGG AAG

_{CCG AAG CAG SWY TGC ATC AT- 3’}

Trop

TropF

_TropR

5’- GAG TTG GAT CGG GCT CAG GA GCG -3’

_{5’- CGG TCA GCC TCT TCA GCA ATG TGC TT -3’}

Friesen et al. (1999)

_{Friesen et al. (1999)}

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Tabela 4

: Relação dos

primers

utilizados para amplificação de parte dos genes nucleares.

Gene

Identificação

_dos

Primers

Sequência dos

primers

T

°°°°

C

Referência

RAG1

1stPCR

2510F

5’- TGG CCA TCC GGG TMA ACA C – 3’

_56/54°C

Li e Ortí (2007)

4090R

5’- CTG AGT CCT TGT GAG CTT CCA TRA AYT T – 3’

Li e Ortí (2007)

RAG1

2ndPCR

2535F

5’- AGC CAG TAC CAT AAG ATG TA – 3’

56/54°C

Li e Ortí (2007)

4078R

5’- TGA GCC TCC ATG AAC TTC TGA AGR TAY TT- 3’

Li e Ortí (2007)

RAG2

1stPCR

164F

5’- AGC TCA AGC TGC GYG CCA T -3’

58/56°C

Presente estudo

RAG2-R6

5’- TGRTCCARGCAGAAGTACTTG -3’

Lovejoy e Collette

₍₂₀₀₁₎

RAG2

2ndPCR

176R

5’- GYG CCA TCT CAT TCT CCA ACA -3’

58/56°C

Presente estudo

Rag2Ri

EgH8=888888=88======HNg

Presente estudo

Myh6

1stPCR

F329

5’- CCG CMT GGA TGA TCT ACA C - 3’

_56/54°C

Li et al.(2007)

A3R1

5’- ATT CTC ACC ACC ATC CAG TTG AA- 3’

Li et al.(2007)

Myh6

2ndPCR

A3F2

5’- GGA GAA TCA RTC KGT GCT CAT CA - 3’

56/54°C

Li et al.(2007)

A3R2

5’- CTC ACC ACC ATC CAG TTG AAC AT - 3’

Li et al.(2007)

Ptr

1st PCR

F458

5’ – AGA ATG GAT WAC CAA CAC YTA CG – 3’

56/54°C

Li et al.(2007)

R1248

5’- TAA GGC ACA GGA TTG AGA TGC T -3’

Li et al.(2007)

Ptr

2nd PCR

F463

5’- GGA TAA CCA ACA CYT ACG TCA A -3’

56/54°C

Li et al.(2007)

R1242

5’- ACA GGA TTG AGA TGC TGT CCA -3’

Li et al.(2007)

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