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Wdr74 is required for blastocyst formation in the mouse.

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Figure 1. Wdr74 is required for blastocyst formation. A. Quantitative RT-PCR analysis of endogenous Wdr74 mRNA during preimplantation development
Figure 2. Gene expression in dsWdr74 morula. A–B. E-cadherin (Cdh1) localization by immunofluorescence marks blastomere cell-cell adhesion as expected in dsGFP morula (A)
Figure 3. Blocking Trp53 permits blastocyst formation in Wdr74-deficient embryos. A–B
Figure 4. Lineage specification in dsWdr74/dsTrp53 blastocysts. A–H. Immunofluorescence localizes Oct4 and Cdx2 to the inner cell mass and trophectoderm, respectively, in dsGFP embryos (A–E)

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