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Potential diagnostic of Branched-Chain Ketoaciduria by HPLC-DAD

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Academic year: 2017

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Table 1. HPLC separation of a standards mixture of L-Leu, L-Ile, and L-Val,  1 mmol L –1 , volume injection: 25 µL
Figure 2. Chromatogram of a standard mixture of L-Val (1), L-Ile (2)  and L-Leu (3) at 0.09 mmol L –1  spiked plasma sample obtained from a  healthy adult volunteer
Table 2. Student’s t test applied to the amino acids analyzed
Table 4. L-Val, L-Ile, and L-Leu concentrations in blood samples of healthy and BCK individuals

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