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Diagnosis of foot and mouth disease by indirect competitive ELISA test in feedlot calves - in Mosul province

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Diagnosis of foot and mouth disease by indirect competitive ELISA test in feedlot

calves - in Mosul province

M. A. Abd-Alhameed and M. Sh. Rhaymah

Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, Iraq

Abstract

Indirect competitive ELISA test used, for detection of non-structural protein (3ABC) in 460 serum samples of infected

animals revealed that 421 samples were positive (91.5%) and 39 samples were negative (8.5%), with 91.5% sensitivity and

28.4% specifity. Blood picture revealed significant (P<0.05) increase in total leukocytic count, mean corpuscular volume,

mean corpuscular hemoglobin and the percentage of granulocytes, and significant decrease in the total erythrocytic count and

packed cell volume.

Available online at

http://www.vetmedmosul.org/ijvs

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1. Gokce G, Gokce HI, Gunes V, Erdogan HM, Citil, M. Alterations in some Haematological and Biochemical parameters in cattle suffering from foot and mouth disease. Turk Vet Sci. 2004;28:723-727. 2. James AD, Rushton J. The economics of foot and mouth disease. Rev

Sci Tech Off Int Epiz. 2002;21:637 – 644.

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2

;

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4'.

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4. Kitching RP. Clinical variation in foot and mouth disease: Cattles. Res Sci Tech Int Epiz.2002a;21:499 – 504.

5. Azab AS, Shahawy MI, Hassos EI. Detection of antibody to foot and mouth disease virus –infection associated (VIA) antigen in cattle. Sera in Nineveah Iraq. Zanco. 1987;5:2.

6. Al-Bana AS, Shony MO. Foot and mouth disease in Iraqi Native Gazella: Virus Isolation, serology and characterization. (master’s thesis). Baghdad: Microbiology, college of veterinary medicine.1988.

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8. Kitching RP, Hughes GJ. Clinical variation in foot and mouth disease: Sheep and goats. Rev Sci Tech Off Int Epiz. 2002b;21:505 – 512. 9. Radostitis OM, Gay CC, Bood DC, Hinchcliff KW. Veterinary

Medicine. A textbook of the disease of cattle, sheep, pigs, and horses. 9th ed. London: W.B. Saunders company; 2000.1059–1066p. 10. Donaldson AI, Hearps A, Alexandersen S. Evaluation of aportable

"real time" PCR machine for FMD diagnosis. Vet Rec. 2001;149: 430.

11. Office International Des Epizooties (OIE). Foot and mouth disease chapter 2.1.1. in manual of standards for diagnostic test and vaccines. 4th ed. OIE, Paris. 2001;77–92p.

12. Robiolo B, Seki C, Fondevilla N, Griger P, Scodeller E, Periolo O, Torre JL, Mattion N. Analysis of the immune response to FMDv structure and non-structural proteins in cattle in Argentina by the combined use pf liquid phase and 3ABC-ELISA tests. 2005. 13. Bergarman I, Malirat V, Neitzert E, Beck E, Panizzutti, N, Sanchez C.

Improvement of a serodiagnostic strategy for foot and mouth disease virus surveillance in cattle under systemic vaccination: a combined system of indirect ELIZA-3ABC with an enzyme linked immuno electrotransfer blot assay. Arch Virol. 2000;145:473–489.

14. Bergmann IE, Mello AP, Neitzert E, Beck E, Gomes I. Diagnosis of persistent apthovirus infection and its differentiation from vaccination response by use of enzyme linked immuno electrotransfer blot analysis with bioengineered non-structural viral antigens. Am J Vet Res.1989;54:825.

15. Thrusfield FM. Veterinary epidemiology. 7th ed. London:

Butterworths; 1986.183p.

16. Diego DM, Brocchi E, Mackay D, Simone DF. The non – structural poly protein 3ABC of foot and mouth disease as a diagnosis antigen in ELISA to differentiate infection from vaccinated cattle. Arch Virol. 1967;142:2021–2033.

17. Clavijo A, Zhou EM, Hole K, Glic B, Kitching P. Development and of a biotinylated 3ABC recombinant protein in a solid – phase competitive ELISA for the detection of antibodies against foot and mouth disease virus. J Virol Meth. 2004;120:217–227.

18. Brousvoort BMDC, Sorensen KJ, Anderson J, Corteyn A, Tanya VN, Kitching RP, Morgan KL. Comparison of 3ABC Enzyme linked Immunosorbant Assay for diagnosis of multiple – serotype foot and mouth disease in a cattle population in an area of endemicity. J. Clin. Microbio. 2004;42:2108-2114.

19. Sorensen KJ, Madsen KG, Madsen ES, Salt JS, Niqindi J, Mackay DKJ. Differentiation of infection from vaccination in foot and mouth disease by the detection of antibodies to the non-structural proteins 3D, 3AB, and 3ABC in ELISA using antigen expressed I bacilovirus. Arch Virol.1989;143:1461–1476.

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