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Combinatorial action of miRNAs regulates transcriptional and post-transcriptional gene silencing following in vivo PNS injury.

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Academic year: 2017

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Figure 1. Protein expression changes and miRNA expression profile following in vivo sciatic nerve injury
Figure 2. miRNA-mRNA target prediction and evaluation. Putative miRNA binding sites detected by RNA 22 are represented on individual mRNAs (not to scale) as squares (repressed in injury) or circles (up regulated in injury) with the maximum folding energy s
Figure 3. miR-138 and miR-709 bind and regulate Sox-2, c-Jun and Egr2. Sox-2, c-Jun and Egr2 transcripts containing multiple target sites for both miRNAs 138 and 709 were cloned into the 3 9 -UTR of luciferase gene in the preport vector
Figure 5. miRNAs mediate transcriptional gene silencing of Egr2. (A). Real-time qRT-PCR of Egr2 transcripts normalized to GAPDH control 6, 24 and 48 hours post-injury as compared to control uninjured nerves
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