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Establishment and Characterization of a Tumor Stem Cell-Based Glioblastoma Invasion Model.

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Fig 1. Characterization of GSS cultures. The three GSS cultures were cultured in serum-free medium as spheroids, which upon trypsination to single cells developed new spheroids (A-C)
Fig 2. Confocal images of spheroid invasion. Confocal z-stacks were recorded on day 0, one hour after implantation of DiI- DiI-labeled spheroids into the brain slice cultures and after 3 and 6 days
Fig 3. Quantitation of area and invasion in confocal images. The spheroid area was measured in the confocal images on day 0, 3 and 6 (T78:
Fig 4. Invasion in the in vitro invasion model. Thin 3 μm sections of spheroids implanted into brain slice cultures were stained by immunostained using anti-human vimentin (A, C, E, G) and CD56 (B, D, F, H) antibodies
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