RNAi-based functional profiling of loci from blood lipid genome-wide association studies identifies genes with cholesterol-regulatory function.

To further confirm the effect of 14-3-3 e on modulating cell migration, control or 14-3-3 e stable cells (clone 1) were transfected with scramble or 14-3-3 e siRNAs and the

HeLa cells were left untreated (Medium, A) or transiently transfected with control RNAi (B) or CK18 RNAi (C).. After 24 h, cells were infected with tis- sue culture trypomastigotes

(C) HeLa cells transfected with either non-targeting siRNA or siRNA targeting CPSF6 were infected with VSV-G-pseudotyped GFP carrying CA mutants that lost the cell cycle independence

Taqman qRT PCR assays (Applied Biosystems) for tyrosinase and MITF was utilized to identify siRNAs that impacted tyrosinase and MITF expression. C) The indicated siRNAs, targeting

HeLa cells were chosen as a model human cell type because they have been used extensively for anthrax toxin entry studies and they are efficiently transfected with siRNAs

(A) Western blot analysis of SETDB1, KAP1 and hnRNP K in TT2 cells transfected with control, Setdb1 or Hnrnpk siRNAs at 24 h post-transfection.. GAPDH was detected as a

siRNAs directed against (A) luciferase or XIAP, (B) cIAP-1, (C) cIAP-2, or (D) survivin were transfected in HeLa cells, and the protein levels of all IAPs were determined in infected

Cells were triple transfected with siRNAs against the FUS, EWS, and TAF15 mRNAs (siFET) or with an unspecific siRNA as control (siControl), and treated with arsenite to induce