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Iron Chelators and Antioxidants Regenerate Neuritic Tree and Nigrostriatal Fibers of MPP+/MPTP-Lesioned Dopaminergic Neurons.

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Academic year: 2017

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Fig 1. Injury and regeneration protocols. A) In vitro axodendritic tree regeneration. Mesencephalic cells at DIV7 were treated for 24 h with 0.5 μM MPP+, followed by treatment for 48 h with chelators or antioxidants.
Fig 2. Total neuritic tree length of control and MPP+-treated TH-positive neurons. Mesencephalic cells (DIV7) were treated for 24 h with 0.5 μM MPP+, after which dopaminergic neurons were identified by TH immunostaining; the cell nucleus was stained with T
Fig 3. Iron chelators restore the axodendritic tree in MPP+-treated DA neurons. Mesencephalic cells in culture were treated as described in the legend to Fig 1A for 24 h with 0.5 μM MPP+ followed by treatment for 48 h with 5 μM DFO, 50 μM DFP or 10 μM DPD
Fig 4. Effect of 8-hydroxyquinoline-based chelators on axodendritic tree restoration. A) Cells were treated for 24 h with 0.5 μM MPP+ and then for 48 h with 50 nM 7MH, 50 nM 7DH or 0.25 μM M30
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