PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression.

(A) Expression plasmid for FLAG-tagged bBST-2A1, bBST-2A2 or bBST-2B was transfected into HEK 293T cells. Cells were dissolved 24 h after transfection and subjected to 12.5%

HeLa 57A cells expressing human TLR2/6 (A) and control cells transfected with vector without insert (B) were stimulated with wild type, D lgt , D lgt ::pGA14- cm , and D lgt

HekT cells were transiently transfected with plasmids encoding the indicated V5-tagged UbcH proteins together with either empty vector or FLAG-tagged Hu, Agm or Mo ISG15 or a

Our results show that CHO-K1 cells sta- bly transfected with the Flag-tagged angio- tensin II receptor bind angiotensin II with an affinity similar to that of cells expressing

(H) Twenty-four hours after transfection with control siRNA or caspase-3 siRNA, HeLa cells were transfected with GFP-empty, GFP-tagged wild-type or mutant p150 glued. Forty-eight

To identify if the kinase activity of Plk1 is involved in the interaction, FLAG-tagged Plk1 and the kinase-defective mutant FLAG-tagged Plk1 KD were expressed in β9γT

FLAG-tagged RIG-I or RIG-I RD expression vector was transfected into HEK293FT cells together with HA-tagged IKK- e (A, B, and E), Myc-tagged TBK1 (C, F), HA-tagged NEMO

In a separate experiment HEK293 cells were transiently transfected with Flag-tagged wild type SIRT6 and cell extracts were prepared and immunoprecipitated with an anti-Flag antibody