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Fibroblasts derived from human pluripotent stem cells activate angiogenic responses in vitro and in vivo.

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Academic year: 2017

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Figure 1. Derivation of EDK and iPDK cells. A. Summary of the differentiation protocol used for the derivation of EDK and iPDK cells from hESCs and hiPSCs
Figure 2. Comparison of secretory protein profiles of EDK, iPDK and BJ cells. A. Cytokine array membranes used to analyze the secretion of angiogenic growth factors and cytokines by EDK, iPDK and BJ cells
Figure 3. Emergence of pericyte markers following differentiation from hESC and hiPSC
Table 2. Flow cytometric analysis of surface markers characteristic of pericytes. % of positive cells Surface marker Antigen Cell type d0 d7 d14 d28 CD140b PDFGRb hESC 10% 30% 65% 90% hiPSC 30% 50% 60% 90% CD73 SH3,  ecto-59-nucteotidase hESC 0.5% 5% 95% 9
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