The homeodomain transcription factor Hoxa2 interacts with and promotes the proteasomal degradation of the E3 ubiquitin protein ligase RCHY1.

HekT cells were transiently transfected with plasmids encoding the indicated V5-tagged UbcH proteins together with either empty vector or FLAG-tagged Hu, Agm or Mo ISG15 or a

Our results show that CHO-K1 cells sta- bly transfected with the Flag-tagged angio- tensin II receptor bind angiotensin II with an affinity similar to that of cells expressing

Dynamic S-palmitoylation of proteins is the addition of palmitic acid by zDHHC palmitoyl transferases (PATs) and depalmitoylation by palmitoyl protein thioesterases (PPTs).. A

In a separate experiment HEK293 cells were transiently transfected with Flag-tagged wild type SIRT6 and cell extracts were prepared and immunoprecipitated with an anti-Flag antibody

(B) Overexpression of KQ Klf5 mutant antagonizes the suppression effect of TGF- β signaling on Sox2 protein expression. Empty pCAGIPuro, Flag-tagged WT and KQ Klf5

C and D HEK293T cells were transfected with the indicated HA-tagged Akt1 phosphorylation site mutants with or without co- transfected FLAG-tagged FKBP51 K352A/R356A (TPR_mut).. After

To decide whether or not c -tubulin 2 differs from c -tubulin 1 in subcellular localization, we examined U2OS cells expressing FLAG-tagged mouse or human c -tubulin 2

The Flag- tagged indicated Zeste deletion mutants were co-expressed in Sf 9 cells with full-length HA-tagged Zeste. The ability to recruit Zeste was tested on a coomassie-stained