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Determination of 2,5-hexanedione in urine by high performance liquid chromatography after derivatization with 2,4-dinitrophenilhidrazine.

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Academic year: 2017

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Figura 1. Reação de derivatização. (1) 2,5-hexanodiona; (2) 2,4-dinitrofeni- 2,4-dinitrofeni-lhidrazina; (3) 5-(2,4-dinitrofenil-hidrazinilideno)-hexan-2-ona
Tabela 2. Parâmetros de validação do método
Figura 2. Gráicos de contornos. Resposta é a área pico de 5-(2,4-dinitrofenil- 5-(2,4-dinitrofenil-hidrazinilideno)-hexan-2-ona,  DHH  (mAU  min)

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