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NURSING WORKLOAD AT A GASTROENTEROLOGY UNIT

1

Marcia Raquel Panunto2 Edinêis de Brito Guirardello3

Panunto MR, Guirardello EB. Nursing workload at a gastroenterology unit. Rev Latino-am Enfermagem 2009 novembro-dezembro; 17(6):1009-14.

One of the biggest challenges nurses face is the need to justify the quantity and quality of staff for care delivery. For this, management instruments are available which help them to determine the staff needed in the nursing team. This descriptive study aims to evaluate the nursing workload at a specialized clinical and surgical gastroenterology unit. To collect data, the Nursing Activities Score (NAS) was used during 30 consecutive days, with 1080 comments, obtained from the records of 149 patients. The mean NAS score was 34.9% and, considering that each point of NAS corresponds to 0.24 hour, on the average, 8.4 hours of nursing care were required within 24 hours. This means that this profile is of patients who demand intermediate and semi-intensive care, which suggests that the NAS study can be used to evaluate the workload of nurses at that unit.

DESCRIPTORS: workload; nursing assessment; nursing staff

CARGA DE TRABAJO DE ENFERMERÍA EN UNA UNIDAD DE GASTROENTEROLOGÍA

Uno de los mayores desafíos enfrentados por el enfermero se refiere a la necesidad de justificar la cantidad y calidad de personal que se necesita en una unidad para la prestación de asistencia. Para eso, dispone de herramientas administrativas que auxilian en la determinación del personal necesario en el equipo de enfermería. Se trata de un estudio descriptivo, que tuvo por objetivo evaluar la carga de trabajo de enfermería en una unidad clínica y quirúrgica, especializada en gastroenterología. Para la recolección de datos, se utilizó el Nursing Activities Score (NAS) por 30 días consecutivos, totalizando 1.080 observaciones, obtenidas de los registros de 149 pacientes. El promedio de la puntuación del NAS fue de 34,9% y, considerando que cada punto del NAS corresponde a 0,24h, fueron requeridas, en promedio, 8,4 horas de enfermería para la asistencia en las 24h. Eso significa que el perfil de esos pacientes corresponde al de aquellos que requieren cuidados intermediarios y semi-intensivos, lo que sugiere que el NAS puede ser utilizado para evaluar la carga de trabajo de enfermería en la referida unidad.

DESCRIPTORES: carga de trabajo; evaluación en enfermería; personal de enfermería

CARGA DE TRABALHO DE ENFERMAGEM EM UMA UNIDADE DE GASTROENTEROLOGIA

Um dos maiores desafios enfrentados pelo enfermeiro refere-se à necessidade de justificar o quantitativo e qualitativo de pessoal para a prestação da assistência. Para isso, dispõe de ferramentas gerenciais que auxiliam na determinação do pessoal necessário na equipe de enfermagem. Trata-se, aqui, de estudo descritivo, que objetivou avaliar a carga de trabalho de enfermagem em uma unidade clínica e cirúrgica, especializada em gastroenterologia. Para a coleta de dados, utilizou-se o Nursing Activities Score (NAS) por 30 dias consecutivos, totalizando 1080 observações, obtidas do registro de 149 pacientes. A média da pontuação do NAS foi de 34,9% e, considerando que cada ponto do NAS corresponde a 0,24h, foram requeridas, em média, 8,4 horas de enfermagem na assistência nas 24h. Isso significa que o perfil desses pacientes corresponde ao daqueles que requerem cuidados intermediários e semi-intensivos, o que sugere que o NAS pode ser utilizado para avaliar a carga de trabalho de enfermagem na referida unidade.

DESCRITORES: carga de trabalho; avaliação em enfermagem; recursos humanos de enfermagem

Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Brazil:

1Basic Scientific FAPESP, Process nº 2007/59957-1. 2RN, e-mail: panunto@gmail.com. 3Associate Professor, e-mail: guirar@fcm.unicamp.br.

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INTRODUCTION

O

ne of the biggest challenges nurses face is

the need to justify the quantity and quality of staff for

care delivery to clients. This process can result in

conflicts, however, as the growing need to decrease

costs and increase nursing service delivery arouses

questions about institutions’ nursing staff (1).

For service management to justify a given

nursing staff, nowadays, nurses have management

tools at their disposal to rank patients in terms of

care complexity level, consequently helping to

determine the nursing staff needed for quality care

delivery. These include instruments developed in Brazil

for adult hospitalized patients(2-6), for residency

nursing(7) and for pediatric patients(8), as well as others

validated for the Brazilian culture, such as the

Therapeutic Intervention Scoring System (TISS-28)(9)

and the Nursing Activities Score (NAS)(10).

Differently from the above instruments, the

NAS assesses the number of nursing hours spent per

patient during one work shift, based on its total score

(10-11)

. It contains 23 items, which cover basic activities

(monitoring, medication, hygiene procedures,

administrative tasks, among others), mechanical

ventilation, cardiovascular, renal, neurological and

metabolic support, besides specific interventions. The

sum of these items shows the time nursing activities

consume in 24-hour patient care delivery. Scores can

range from zero to 100 (%) or more, which can mean

that more than one nursing professional was needed

to deliver care to the patient on a given day(11).

Although the NAS was constructed for

application to patients from critical care units, it is

considered useful to assess nursing workload at

tertiary and quaternary hospital units, where clients’

care complexity level is high. It should be reminded

that the NAS score does not depend on patient

severity, as its construction and validation were based

on nursing activities(11).

The Patient Classification System (PCS)(2,4)

has been used for staff dimensioning at hospitalization

units, classifying patients in care groups or categories.

While this type of tool identifies the patient’s level of

complexity, the NAS permits measuring the workload

in terms of direct and indirectly related nursing care

hours. However, studies using the NAS have been

applied to ICU patients, evidencing its reliability to

assess workloads for patients who require intensive

care(12-15).

The choice of a patient classification instrument

to identify the workload should not only be based on

its specific indication, but should also take into account

an institution’s care delivery dynamics and complexity.

In view of difficulties to apply an instrument

that permits classifying patients into care complexity

levels, this research aims to measure the nursing

workload at a clinical and surgical unit like

gastroenterology.

METHOD

This descriptive study was carried out at a

gastroenterology unit of a teaching hospital in the

interior of São Paulo state. The unit is subdivided into

two nursing wards, gastroclinic and gastrosurgery,

totaling 36 beds, eight for the gastroclinic and 28 for

gastrosurgery.

To collect data, the NAS was used to measure

the nursing workload, besides a form to characterize

the patients’ demographic and clinical data. For the

study, all adult patients attended at the unit during

the research period were considered.

Approval for the research was obtained from

the institution’s Ethics Committee (Process No 758/

2007), requesting dispensation from the signing of

the free and informed consent term (FICT). This

request was justified to the Ethics Committee as the

assessment of patient care demands is considered a

daily nursing activity and because the patient was

not submitted to any other procedure in function of

the application of the instrument.

Before data collection, the instrument was

submitted to a 15-day pretest to allow the researcher

to become familiar with the tool and adapt some of

its aspects to the patients’ profile at the unit. The

researcher herself collected the data during 30

consecutive days, from April 14th to May 14th 2008.

Data were collected based on patient assessment,

information in nursing notes and patient files, as well

as verbal information provided by the nursing team

which was not recorded in the files.

The collected data were transferred to a

Microsoft® Excel worksheet and analyzed through

SPSS® 16.0 for Windows. Frequency tables were

elaborated for the categorical variables and descriptive

statistics (mean, standard deviation, median,

minimum and maximum) for the continuous variables.

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between the posts and the categorical variables, and

Fisher’s exact test was applied when expected levels

were below five. Mann-Whitney’s non-parametric test

was used to compare the wards in terms of categorical

variables. The significance level was set at 5%, i.e. p

≤0.05.

RESULTS

The NAS was applied during 30 consecutive

days to the 36 beds at the gastroenterology unit

(gastroclinic and gastrosurgery nursing wards),

totaling 1080 observations, obtained from 149 patients’

records. To measure the workload at the unit, the

researcher decided to consider the bed, independently

from the subject occupying it. Hence, the collected

data followed bed occupation dynamics.

In the group of 149 patients, 56.4% came

from gastrosurgery and 43.6% from the gastroclinic.

Most cases referred to elective hospitalization. Mean

age at the unit was 50 years (min=18 and max=90),

and patients’ mean hospitalization time was 7 days

(min=1 and max=67). In this group, 59.7% were men

and 40.3% women.

Data indicated that, at the gastroclinical ward,

male patients with liver diseases predominated when

compared with the gastrosurgery ward. On the other

hand, in gastrosurgery, most patients were

hospitalized for less than five days and were submitted

to surgical treatment.

T h e w o r k l o a d a c c o r d i n g t o t h e N A S ,

including mean and median scores for the unit and

w a r d s , i s s h o w n i n Ta b l e 1 . M i n i m u m s c o r e s

equalling zero mean that the bed was empty during

a data collection day.

t i n

U N Mean SD Median Minimum Maximum pvalue*

c i n il c o r t s a

G 540 37.2 23.9 35.6 0 136 0.001 y r e g r u s o r t s a

G 540 32.6 20 29.9 0 113 l

a t o

T 1080 34.9 22.2 33.7 0 136

Table 1 – NAS workload score at gastroenterology unit (%). Campinas, 2008

*Mann-Whitney

The observation of mean NAS scores per bed

revealed that four beds, with generators, located in

two rooms at the gastroclinical ward, obtained the

highest mean workload scores, ranging between 44.9

and 68.3%, when compared with other beds at the

unit. On the other hand, the four beds at the

gastrosurgery ward, with the same characteristic,

scored equal or lower than other beds at this ward.

As the NAS permits identifying nursing

activities during 24-hour patient care, the recording

frequency of instrument items and subitems can be

calculated (Table 2).

Table 2 - Frequency of item and subitem performance of Nursing Activities Score. Campinas, 2008

s m e t i b u s d n a s m e t

I n %

n o i t a r t i t d n a g n i r o t i n o M . 1 ) s t p 5 . 4 ( . e c n a l a b d i u l f f o n o i t a l u c l a c d n a n o i t a r t s i g e r r a l u g e r , s n g i s l a t i v y l r u o H . a

1 840 77.8

) s t p 1 . 2 1 ( . e r o m r o s r h 2 r o f e v i t c a r o n o i t a v r e s b o s u o u n i t n o c d n a e d i s d e b t a t n e s e r P . b

1 83 7.7

) s t p 6 . 9 1 ( . e r o m r o s r h 4 r o f e v i t c a d n a e d i s d e b t a t n e s e r P . c

1 5 0.5

) s t p 3 . 4 ( . s n o i t a g i t s e v n i l a c i g o l o i b o r c i m d n a l a c i m e h c o i b , y r o t a r o b a L .

2 276 25.6

) s t p 6 . 5 ( . d e d u l c x e s g u r d e v i t c a o s a v , n o i t a c i d e M .

3 916 84.8

s e r u d e c o r p e n e i g y H . 4 ) s t p 1 . 4 ( . s e r u d e c o r p e n e i g y h g n i m r o f r e P . a

4 812 75.2

) s t p 5 . 6 1 ( . s r h 2 n a h t e r o m k o o t s e r u d e c o r p e n e i g y h f o e c n a m r o f r e p e h T . b

4 90 8.3

) s t p 0 . 0 2 ( . s r h 4 n a h t e r o m k o o t s e r u d e c o r p e n e i g y h f o e c n a m r o f r e p e h T . c

4 2 0.2

) s t p 8 . 1 ( . ) e b u t c i r t s a g t p e c x e ( ll A -s n i a r d f o e r a C .

5 244 22.6

g n i n o i t i s o p d n a n o i t a z il i b o M . 6 ) s t p 5 . 5 ( . s r h 4 2 r e p s e m i t e e r h t o t p u ) s ( e r u d e c o r p g n i m r o f r e P . a

6 71 6.6

) s t p 4 . 2 1 ( . y c n e u q e r f y n a , s e s r u n o w t h t i w r o , s r h 4 2 r e p s e m i t 3 n a h t y l t n e u q e r f e r o m ) s ( e r u d e c o r p g n i m r o f r e P . b

6 45 4.2

) s t p 0 . 7 1 ( . y c n e u q e r f y n a , s e s r u n e r o m r o e e r h t h t i w e r u d e c o r p g n i m r o f r e P . c

6 3 0.3

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Table 2 - Continuation s m e t i b u s d n a s m e t

I n %

t n e i t a p d n a s e v i t a l e r f o e r a c d n a t r o p p u S . 7 ) s t p 0 . 4 ( . t f i h s y n a n i r h 1 t u o b a r o f n o i t a c i d e d ll u f g n i r i u q e r t n e i t a p r o s e v i t a l e r r e h t i e f o e r a c d n a t r o p p u S . a

7 881 81.6

) s t p 0 . 2 3 ( . y n a n i e r o m r o s r h 3 r o f n o i t a c i d e d ll u f g n i r i u q e r t n e i t a p r o s e v i t a l e r r e h t i e f o e r a c d n a t r o p p u S . b

7 46 4.3

s k s a t l a i r e g a n a m d n a e v i t a r t s i n i m d A . 8 f o e g n a h c x e l a n o i s s e f o r p , s n o i t a n i m a x e g n i r e d r o , a t a d l a c i n il c f o g n i s s e c o r p s a h c u s s k s a t e n i t u o r g n i m r o f r e P . a 8 ) s t p 2 . 4 ( . n o i t a m r o f n

i 606 56.1

) s t p 2 . 3 2 ( . t f i h s y n a n i s r h 2 t u o b a r o f n o i t a c i d e d ll u f g n i r i u q e r s k s a t l a i r e g a n a m d n a e v i t a r t s i n i m d a g n i m r o f r e P . b

8 328 30.4

n i e m i t e h t f o e r o m r o s r h 4 t u o b a r o f n o i t a c i d e d ll u f g n i r i u q e r s k s a t l a i r e g a n a m d n a e v i t a r t s i n i m d a g n i m r o f r e P . c 8 ) s t p 0 . 0 3 ( . t f i h s y n

a 5 0.5

y n a y b n e g y x o y r a t n e m e l p p u s ; n o i t a l i t n e v d e t s i s s a / n o i t a l i t n e v l a c i n a h c e m f o m r o f y n a : t r o p p u s y r o t a r i p s e R . 9 ) s t p 4 . 1 ( . d o h t e

m 74 6.9

) s t p 8 . 1 ( . s y a w r i a l a i c i f i t r a f o e r a C . 0

1 45 4.2

) s t p 4 . 4 ( . n o i t c n u f g n u l g n i v o r p m i r o f t n e m t a e r T . 1

1 298 27.6

) s t p 2 . 1 ( . e s o d d n a e p y t d r a g e r s i d , n o i t a c i d e m e v i t c a o s a V . 2

1 21 1.9

) s t p 5 . 2 ( . s e s s o l d i u l f e g r a l f o t n e m e c a l p e r s u o n e v a r t n I . 3

1 0 0

) s t p 7 . 1 ( . g n i r o t i n o m m u i r t a t f e L . 4

1 0 0

) s t p 1 . 7 ( . s r h 4 2 f o d o i r e p t s a p e h t n i n o i t a t i c s u s e r y r a n o m l u p o i d r a C . 5

1 3 0.3

) s t p 7 . 7 ( . s e u q i n h c e t s i s y l a i D . s e u q i n h c e t n o i t a r tl i f o m e H . 6

1 10 0.9

) s t p 0 . 7 ( . t n e m e r u s a e m t u p t u o e n i r u e v i t a t i t n a u Q . 7

1 422 39.1

) s t p 6 . 1 ( . e r u s s e r p l a i n a r c a r t n i f o t n e m e r u s a e M . 8

1 0 0

) s t p 3 . 1 ( . s i s o l a k l a / s i s o d i c a c il o b a t e m d e t a c il p m o c f o t n e m t a e r T . 9

1 6 0.6

) s t p 8 . 2 ( . n o i t a t n e m il a r e p y h s u o n e v a r t n I . 0

2 49 4.5

) s t p 3 . 1 ( . e t u o r l a n i t s e t n i o r t s a g r e h t o r o e b u t c i r t s a g h g u o r h t g n i d e e f l a r e t n E . 1

2 64 5.9

) s t p 8 . 2 ( . t i n u e r a c e v i s n e t n i e h t n i ) s ( n o i t n e v r e t n i c i f i c e p S . 2

2 41 3.8

) s t p 9 . 1 ( . t i n u e r a c e v i s n e t n i e h t e d i s t u o s n o i t n e v r e t n i c i f i c e p S . 3

2 199 18.4

In items 1, 4, 6, 7 and 8, only one subitem can be scored

DISCUSSION

Study data appoint the predominance of male

patients, submitted to surgical treatment and

hospitalized for less than five days. The greatest

hospitalization turnover was found at the

gastrosurgical ward.

At that ward, most patients were admitted

for surgical patients, but patients for clinical treatment

were also attended, as some of these, in the late

post-operative period, are hospitalized for clinical

compensation. At the gastroclinic, on the other hand,

these percentages did not differ because the unit

offers beds for clinical as well as surgical care.

According to the NAS, the mean workload at

the gastroenterology unit was 34.9%. The comparison

between both wards showed a difference, with a

higher score for the gastroclinical than for the

gastrosurgery ward. These results could not be

compared with other studies, however, as the

application of this instrument has been restricted to

intensive care units, but some of the reasons for these

differences can be due to the level of dependence

and clinical procedures performed. Studies carried

out at ICU appoint a higher mean workload(10,12-15) than

what was found in this research, although one of those

studies(14) reported a lower minimum NAS score than

the mean score found at the gastroenterology unit.

The gastroclinical ward obtained the highest

mean workload scores, for beds with generators as

well as for other beds at the ward, which means that

these beds were occupied by patients with greater

nursing care demands. It is interesting to highlight

that, in gastrosurgery, some of the beds not equipped

for care delivery to patients with high care demands

scored the same or higher than beds with generators.

The most frequent NAS items were: 1a. Hourly vital signs, regular registration and calculation of fluid balance; 3. Medication, vasoactive drugs excluded; 4a. Performing hygiene procedures; 7a. Support and care of either relatives or patient requiring full dedication for about 1 hr in any shift and 8a. Performing routine tasks.

Unscored items are relatived to activities

performed at a specialized intensive care unit(14-15),

according to expectations for the gastroenterology

patient profile.

To obtain NAS workload results comparable

with the total nursing hours recommended by the

(5)

according to the type of care the patient demands,

the NAS score needs to be transformed into hours.

Each NAS point is equivalent to 0.24hr(14) and,

on the average, 34.9% of the workload at the

gastroenterology unit was measured, 8.4 hours of

nursing service were delivered during 24-hour care.

In view of recommendations established in COFEN

resolution No 293/2004(16), the obtained score

evidences a profile of patients demanding

intermediary and semi-intensive care, suggesting that

the NAS can be used to assess the nursing workload

at that unit.

CONCLUSIONS

This research permitted measuring the

nursing workload at a specialized clinical and surgical

gastroenterology care unit. The workload, according

to the NAS, corresponded to 34.9% for the unit, with

differences between the nursing wards, i.e. 37.2%

for the gastroclinic and 32.63% for the gastrosurgical

ward.

The total score resulted in an average 8.4

hours of nursing care during 24 hours, corresponding

to the profile of patients demanding intermediary and

semi-intensive care. Although the NAS is applicable

to ICU, its utility to determine the nursing workload

at the study unit could be demonstrated.

In view of these results, the importance of

nurses assessing the nursing workload at their unit is

highlighted, using instruments for this goal, either to

make the nursing work process more oriented to

patient demands or to help nurses to administer the

staff in hospital service management.

As this was the first experience of using the

NAS at a clinical and surgical unit, the researchers

recommend its application in other studies with the

same patient profile and hospital reality, as well as its

validation for other than intensive care units.

REFERENCES

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5. Hokama CSM, Serrano CDBH. Sistema de classificação de pacientes (SCP) – ENFSCORE. In: Bork AMT, organizador. Enfermagem de excelência: da visão à ação. Rio de Janeiro (RJ): Guanabara Koogan; 2003. p. 101-10.

6. Martins EAP, Haddad MCL. Validação de um instrumento que classifica os pacientes em quatro graus de dependência do cuidado de enfermagem. Rev Latino-am Enfermagem 2000 abril; 8(2):74-82.

7. Dal Ben LW. Instrumento para dimensionar horas diárias de assistência de enfermagem residencial [dissertação]. São Paulo (SP): Escola de Enfermagem/USP; 2000.

8. Dini AP. Sistema de classificação de pacientes pediátricos: construção e validação de instrumento [dissertação]. Campinas (SP): Universidade Estadual de Campinas; 2007. 9. Nunes B. Tradução para o português e validação de um

instrumento de medida de gravidade em UTI: Therapeutic Intervention Scoring System-28 (TISS-28) [dissertação]. São Paulo (SP): Escola de Enfermagem/USP; 2000. 10. Queijo AF. Tradução para o português e validação de um instrumento de medida de carga de trabalho de enfermagem em unidade de terapia intensiva: Nursing Activities Score (NAS) [dissertação]. São Paulo (SP): Escola de Enfermagem/ USP; 2002.

11. Miranda DR, Nap R, Rijk A, Schaufeli W, Iapichino G. Nursing Activities Score. Crit Care Med 2003; 31(2):374-82. 12. Balsanelli AP, Cunha ICKO, Whitaker IY. Estilos de liderança de enfermeiros em unidade de terapia intensiva: associação com perfil pessoal, profissional e carga de t rabalho . Rev. Latino-Am. Enfermagem [periódico na Internet]. 2009 Fev [citado 2009 Jun 02] ; 17(1): 28-33. Disponível em: http://www.scielo.br/scielo.php? s c r i p t = s c i _ a r t t e x t & p i d = S 0 1 0 4 - 1 1 6 9 2 0 0 9 0 0 0 1 0 0 0 0 5 & l n g = p t . d o i : 1 0 . 1 5 9 0 / S 0 1 0 4 - 1 1 6 9 2 0 0 9 0 0 0 1 0 0 0 0 5 .

13. Sousa CR, Gonçalves LA, Toffoleto MC, Leão K, Padilha KG. Preditores da demanda de trabalho de enfermagem para idosos internados em unidade de terapia intensiva. Rev. Latino-Am. Enfermagem [periódico na Internet]. 2008 Abr [citado 2009 Jun 02] ; 16(2):218-23. Disponível em: http:// www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-11692008000200008&lng=pt. doi: 10.1590/S0104-1 10.1590/S0104-1 6 9 2 0 0 8 0 0 0 2 0 0 0 0 8 .

14. Conishi RMY. Avaliação do NAS – Nursing Activities Score – como instrumento de carga de trabalho de enfermagem em uma UTI geral adulto [dissertação]. São Paulo (SP): Escola de Enfermagem/ USP; 2005.

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de enfermagem em uma unidade de terapia intensiva de adultos no primeiro dia de internação [dissertação]. São Paulo (SP): Escola de Enfermagem/USP; 2006.

16. Conselho Federal de Enfermagem [Página na internet]. Resolução 293/2004. Fixa e estabelece parâmetros para o

dimensionamento de pessoal de enfermagem nas unidades assistenciais das instituições de saúde e assemelhados [acesso em 29 fev 2008]. Disponível em: http:// w w w . p o r t a l c o f e n . c o m . b r / 2 0 0 7 / m a t e r i a s . a s p ? A r t i c l e ID=7121&sectionID=34

Imagem

Table 2 - Frequency of item and subitem performance of Nursing Activities Score. Campinas, 2008
Table 2 - Continuation smetibusdnasmetI n % tneitapdnasevitalerfoeracdnatroppuS.7 )stp0.4(.tfihsynanirh1tuobarofnoitacidedllufgniriuqertneitaprosevitalerrehtiefoeracdnatroppuS.a7 8 8 1 8 1

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Ao analisar o desempenho do Nursing Activities Score para a medida prospectiva de carga de trabalho de enfermagem em Unidade de Terapia Intensiva e comparar os valores do

Não obstante a reconhecida necessidade desses serviços, tem-se observado graves falhas na gestão dos contratos de fornecimento de mão de obra terceirizada, bem

Tradução para o português e validação de um ins- trumento de medida de carga de trabalho de enfermagem em Unidade de Terapia Intensiva: Nursing Activities Score (NAS)

Tradução para o português e validação de um ins- trumento de medida de carga de trabalho de enfermagem em Unidade de Terapia Intensiva: Nursing Activities Score (NAS)

(...) Uma boa atriz, uma boa mentirosa, mas não, na verdade, uma pessoa muito boa.” (KUSHNER, 1994, p. 35) Provavelmente tão mundana quanto o desejo de um ator de

Outras possíveis causas de paralisia flácida, ataxia e desordens neuromusculares, (como a ação de hemoparasitas, toxoplasmose, neosporose e botulismo) foram descartadas,