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Enigma prevents Cbl-c-mediated ubiquitination and degradation of RETMEN2A.

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Academic year: 2017

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Figure 1. Enigma interacts with Cbl-c. (A) HEK293T cells were transfected with GST, or GST-Cbl-c with and without untagged human Enigma as indicated above each blot
Figure 2. Cbl binds RET and MEN2A kinases. (A) HEK293T cells were transfected separately with pJ7V plasmids encoding each of the following four RET kinase isoforms: RET 9, RET 51, and their MEN2A (C634R) mutant isoforms
Figure 4. Enigma blocks Cbl-c-mediated RET9MEN2A ubiquitination. (A) HEK293T cells were transfected with RET9MEN2A along with GST- GST-Cbl-c, FLAG-Enigma or FLAG-EnigmaDLIM2-3 alone and in combination as indicated above the blots
Figure 5. Comparison of survival outcome between Enigma and Cbl-c expression in high RET expressing breast cancers

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