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Time-Dependent Increase in Network Response to Stimulation.

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Fig 1. In vitro cultures plated on microelectrode arrays are spontaneously active. (a) Cortical neurons extracted from embryonic mice were dissociated and plated on 64-channel MEAs
Fig 2. Parameters for probing stimulation and training signal. (a) Probing stimulation consisted of ± 900 mV bi-phasic pulses administered at a frequency of 0.5 Hz
Fig 3 shows the activity response of eight active electrodes from a representative network that received training
Fig 4. Network response to stimulation was significantly different for trained networks compared to controls
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