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EXPRESSION OF MOUSE BETA DEFENSIN 2 IN ESCHERICHIA COLI AND ITS BROAD-SPECTRUM ANTIMICROBIAL ACTIVITY Tianxiang Gong

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Academic year: 2019

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Figure 1. Schematic representation of the expression vector, pET32a-mBD2. The cDNA for mature mBD2 were amplified using PCR from the pcDNA3.1(+)-mBD2 plasmid
Figure 2. Expression of fusion protein TrxA-mBD2 in E. coli Rosseta-gami (2)/pET32a-mBD2 cells and Tricine-SDS-PAGE analysis of enterokinase digested
Figure 4. Antifungal activity of rmBD2 using the colony counting assay. Fungal cells were grown overnight in medium YPG
Figure 5. Antiviral activity of rmBD2 using the MTT assay.

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