Genome-wide RNAi screen identifies novel host proteins required for alphavirus entry.

mosquitoes for infection with chikungunya virus (CHIKV; genus Alphavirus ) and yellow fever virus (YFV; genus Flavivirus ), suggesting a molecular basis for previous observations

Yleiskielen mukainen potentiaali on vain yksi lienee-muodon kielenkäytössä esiintyvistä käyttötavoista: toisinaan se esiintyy irrallaan olla-verbin paradigmasta, kuten lienee teke-

The use of reverse genetic tools such as RNA interference (RNAi) therefore represents an alternative strategy to identify host proteins that might be important for T. cruzi

When treated with PSD or LPS, MyD88 knockout macrophage cells secreted significantly less IL-6 compared to the level of IL-6 detected in MyD88 positive macrophages (Fig. 3A and

First, in the screen carried out using standard plates (i.e., without adding AP-2 siRNA), knockdowns with a similar phenotype to the clathrin knockdown, defined as those with values

rrf-3 , we reproducibly detected the published phenotypes for 34 genetic mutants for which no RNAi phenotype was reported in the N2 screens (nine essential genes, 21 with

To address this, we took advantage of the tractable genetic model Caenorhabditis elegans expressing expanded polyglutamines (polyQ) and performed a genome-wide RNA interference

To test whether the impact of radiation on promoter methylation was consistent with the results of the peripheral blood, Rad23b and DNA-damage-inducible transcript 3 (Ddit3) genes

Uninfected (top) and infected (bottom). B ) Fluorescence increase with different MOIs of SINV. C6/36 cells were transfected with pBG446 and infected for 36 hrs at the indicated