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Micromass cultures are effective for differentiation of human amniotic fluid stem cells into chondrocytes

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Academic year: 2019

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Figure 2 - Adherent AFMSCs on plastic showing fibroblast-like morphology after 10 days in culture (400X).
Figure 3 - Capacity to differentiate: A) Osteogenic differentiation on plastic shown after staining with Alizarin red; B) Chondrogenic differentiation after staining with Alcian blue; C) Adipogenic differentiation shown by oil red O.
Figure 6 - Western blotting showing positivity for collagen type II in culture medium with TGF-b3 after 21 days.
Figure 7 - Immunohistochemistry. A and B) Negative control; C and D) Positive immunohistochemical staining for collagen type II;

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