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Cathepsin inhibition-induced lysosomal dysfunction enhances pancreatic beta-cell apoptosis in high glucose.

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Academic year: 2017

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Figure 1. Inhibition of cathepsins B and L triggers apoptosis in INS-1 cells. (A) INS-1 cells were treated with E64d (E, 20 μg/mL) and pepstatin A (P, 20 μg/mL) in 11 mM glucose medium for 48 hr, and caspase activation was monitored by western blotting usi
Figure 2. Inhibition of cathepsins B and L increases caspase-dependent apoptosis under glucotoxicity
Figure 3. Inhibition of cathepsins B and L leads to LC3 accumulation. (A) Activity of cathepsins B and L in 30 mM glucose with E64d (E, 20 μg/mL), cathepsin B inhibitor (CBi, 20 μM) or cathepsin L inhibitor (CLi, 20 μM) was measured at 24 hr, 48 hr and 72
Figure 4. Cathepsin B- and L-knockdown with siRNA enhances apoptosis and impairs autophagy
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