comprimento de onda (nm)

!"# $%& ' (#

#) * " " + ,

- .%%

/ #

0_%¹²3,,4_%^%5 6 7 " " +

8 * 9 :

(# * # ; #

8 # 3,,4% 0 % *

< # ; . 6 7

# 3,,4% * #<

# '& 8 ' " #

# =

(# * :

6 7& : #

6 7 !"# $> # "

6 7 #

650 700 750 800 0

400 800 1200 1600

2000 b

0 500 1000 1500 2000 20

40 60 80 100 120 140 160

intensidade (u.a)

tempo (min)

a

intensidade (u.a)

comprimento de onda (nm)

!"# $>& & # 3,,4_% ? @A # (# 0 %

6 7 ? % A # &A# .

#

, 5 (# # ' * # :

# 5 # (#: # ϕ & B

# # ϕ *

$

, 6 7 #< # (# ϕ (#

# # C (# # #

# # ) 6² * # 0%12

3,,4%%5

D (#: # >E =

ϕ τ τ $.&

(# τ * # τ *

(#

τ ≅ ε $ &

(# ε * "

4←4 , # ϕ # τ

F# ε , 3,,4% 4←4 #

; .%%

< (#: # ϕ (#

9 6 7 ϕ & =

ϕ ϕ τ ε τ ε τ τ $G&

(# * 3,,4% * H 9 ?ε *

# .%% ?ε * (# 9 6 7

, # < ϕ ϕ # *

# =

ϕ ϕ $>&

(# # "

# 6 7

# 3,,4_% H

# # * " # I 9 8τ

# % ± %& (#

J # < #< $ ± $& !"# $ &

0 10 20 30 40

1 10 100 1000 10000

ττττ (ns):

4.0 3.0

contagens

tempo (ns)

[TPPS4] = 10 µµµµM pH 4.0 [NaCl] = 0

[NaCl] = 0.4 M T = 20 min

!"# $ &7 * # 3,,4_% ? @A 0 % #

6 7 ? % A

6 3 1 <J (#:

# # ϕ & ϕ & 6 7 # *

(# J $G ϕ ϕ $> ϕ ϕ # 3,,4% ? E µA 6 7 ? $A

D (#: # ϕ& 3,,4_% 6 7 ? $ A

; : λ ? .%% # λ ?

.

ττττ A644nm ^{ϕϕϕϕ ϕϕϕϕ ϕϕϕϕ ϕϕϕϕ}

% %G %

$ $ %

.

.E

.

, 5 (# ϕ ϕ * "#

/ # (# (#

# ϕ #< # 5) * #

8 # # =

D # τ (# * # K

1 D # 4 #

"

"# # # !"# $> & 6 "# $ *

# & # 3,,4_% ? E µA

6 7 ? 1 A # λ ?

% $ # " " 0 # & λ?

" " + # &

0 500 1000 1500 2000 2500

0,0 0,2 0,4 0,6 0,8

1,0 b c

a

intensidade (u.a)

tempo (s)

!"# $ &, * # . # &

# 3,,4_% ? E µA 6 7 ? 1 A

# λ? % $ # & λ? # &

8 ' (# #

69 # ' 1

" " = " " 0 1 (# " " +

# $ # * ; L# #

* #

8 L# # # 9 % $

* "# (# =

! " #τ $

8 L# 1 $ # ' < # (#

! " #τ

8 L# (# ) #

' $. ±$ & ; (# " " 0

$$±% & # ' ±$ & # ) "#

(# " " + % ±% & /

# ' # ϕ #<

(M " " 0%12

3,,4%%5

8 (# " " #

(#: # * H # 1 %.

! "# #!$

(# 6 7 # " "

0%123,,4%%5 < H #< DN4&

DN4 * (# # )# H

J :

6 # 6 7 # H

- !"# $ 1 # & ' <

< # * * #

# H (# 9

6 7 , H L; "#

# (# " " 0

H * < # (#

- # ; < !"# $ 1 # & "#

DN4 * # #) # !"# $ 1 # & :

* !"# $ $

400 450 500 550 600

0 75 150 225

c

b

a

comprimento de onda (nm)

in te ns id ad e (u .a )

!"# $ 1& & H #< 3,,4_% ? µA 0 % = &

- # 6 7K & " " 0 & " " + 6 7 ? % A

0 10 20 30 40 50 60 70 80

0.0 0.2 0.4 0.6 0.8 1.0

intensidade normalizada

tempo (min)

402 nm 506 nm

!"# $ $&B : H # (# 3,,4_% ? @A 0 %

6 7 ? % A λ? % 1 E .

8 H "# I #

, O # >>

" " + 3,,4% , * #

I " " + 8 #

# < H

# 9

" " 6 7 '

" " 0 < !"# $ 1 # (#

# #) " " + !"# $ 1 #

! # ; ≈ % < &

H " " " + !"# $ %&

!"# $ E : DN4

# J 3,,4_% J 6 7 (#

) 1 # #

6 7 #

450 500 550

0 2000 4000 6000 8000

intensidade (u.a)

comprimento de onda (nm)

!"# $ %& H $ & 3,,4_% ? @A # (# 0

% 6 7 ? % A #

0 5 10 15 20 25 30 0.0

0.2 0.4 0.6 0.8 1.0

tempo (min)

[NaCl] = 0.2 M [NaCl] = 0.3 M [NaCl] = 0.4 M

!"# $ E&, * H λ? E # (# 3,,4_% ? E@A

0 % # 6 7

6 !"# $ . *

* * 9 H , 5 (#

# DN4 * (#

% 1 H *

(# (# ; #

0 5 10 15 20 25 30 35

0,0 0,2 0,4 0,6 0,8 1,0

I/I max

Tempo (min)

espalhamento absorção

!"# $ .&A# H # (# 3,,4_%

? E @A 0 % 6 7 ? 1 A #

& '

/ # # (#

" " 3,,4_% # * < $

' 6 7 # 3,,4_% #< # #

) 6² * # "#

" " 0 (# ## " " +

J " " 3,,4_%

E # # < 3,,4_% #

(# H " # $ ^5% A :

J " " ; 0C$ &

> #

" " + 0 3,,4% J P E ^5E A , * #

(# " " J

3,,4% 5.A& 0 *% G&

A (# # #

# J H > 6 H # (#

" " 3,,4% (#

" " + J 3,,4% (# #

* " " 0 (#

" ; ; & 6 * (# ##

" ; J (#

## ;

, ; # (#

3,,4% # " "

" " 0 (# # #) " " +

8 # > (# 3,,4_% ? ^5% A

) " " +* (# " " 0

J " "

## (# # ) + 0

## L; E (#

J " " # ##

)

8 " " + 3,,4_%

; H , O #

# * 4 " " + 3,,4% >>

* * < # ) # #L #

3,,4_% J 0 - & *

H # # " ; , O 5

# >> 8 # ) #

# * ; 3,,4_% # < * *

69 ) #

" " 0 # # # ) " " +

B (# * % $ H#

# (# " " 0

Q * " "

I " " # " " +

# * .. (# ; <

- .%% " " +

!"# E & (# # " " #

* " EE CλC E

# ' " (# " " + 3,,4% I

" " * -

" " ; H

, < # J *

- * # " " !"#

$%&

B

dt K dA dt

dA_{707 644}

−

=

(# _{% %} : .%% & *#

, #

[ ]

dt TPPS ld dt

dA ₄

644 =ε644

[ ]

dt TPPS ld

dt K

dA ₄

707 =− ε644

[ ] [ ]

{

}

[

]

[

]

l K t

A₇₀₇()= ε_{644 4 0} − ₄ = ε_{644 4} =ε_{707 4}

(# ε ε% % .%% '(()$

'(()$ * J ; -

'(()$ + * -

" " , (# : % %*

- " "

B * J

I * " " " " + 3,,4% E . *.

> Q < H D R "H # I

" " 0 % # < DN4 E µA 3,,4%

J 6 7= 1 A $A % A

5 (# DN4

L 1 % $&

, ) & -

, ) + & -+ + & +- & + - & + - & + %

(# & * # -+ - * # *

" " - # - _{+ +} J " "

- " " 6 (# ;

" " - " " * "#

7 # < '_DN4 '_DN4

I " " " " +=

? 'DN4F'DN4 .

, J ?

1 ±1 4# I " " # #

H " " 0 (#

# I " " * # .&

8 (# 1 )

" " + 9 * (# DN4 J

(# 9 " "

- " " (#

DN4 # # I * " " 3,,4%

; (# 9 " "