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No evidence for AID/MBD4-coupled DNA demethylation in zebrafish embryos.

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Academic year: 2017

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Fig. 1. Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs
Fig. 2. No demethylation at M-DNA or randomly chosen loci was detected by the M-DNA-injection into eggs
Fig. 3. M-DNA did not induce the expression of aid or apobec2a. (A) The expression levels of AID/Apobec family genes at the indicated time points were quantified by qPCR, and these expression levels in wild-type embryos (white rectangles) were compared wit
Fig. 4. Zebrafish MBD4 lacks a MBD and mbd4 MO did not interfere splicing of transcripts
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