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A differential polypeptide approach to fight human fungal pathogens

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Academic year: 2021

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Figure I.1: Experiment which led to BLAD discovery back in 1991, as described in [22] and [23]
Figure  II.1:  Electrophoretic  analysis  in  1%  (w/v)  agarose  gel.  (A)  Amplification  with  the  t7  set  of  primers  of pET151  D-TOPO® containing  the  gene  that codifies for the polypeptide BLAD
Figure II.3: SDS-PAGE analysis. Recombinant BLAD expression at 0h with [1] and without IPTG [2]; 4h with [3]
Figure II.4: SDS-PAGE analysis. Purification under  denaturing  condition  of  recombinant  BLAD  using  the  Ni-NTA  agarose  columns
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