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Transcriptomic Changes Due to Cytoplasmic TDP-43 Expression Reveal Dysregulation of Histone Transcripts and Nuclear Chromatin.

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Academic year: 2017

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Fig 1. Transcriptomic analysis of Δ NLS-hTDP-43 expressing mice. (A), Comparison of normalized mean of read counts with log 2 (fold change) shows no bias for highly expressed genes to be categorized as differentially expressed
Fig 2. Nuclear and cytosolic fractionation of Δ NLS-hTDP-43 expressing mice. Subcellular fractionation followed by immunoblot protein analysis reveals a similar level of nuclear total (human and mouse, h+m) TDP-43 levels between bigenic (+/+) and control (
Fig 3. Transcriptomic comparison between Δ NLS-hTDP-43 overexpression and TDP-43 ASO knockdown
Fig 4. Histone transcript dysregulation in Δ NLS-hTDP-43 mice. (A), Comparison of multimapped read fold change in canonical and variant histones shows a significant upregulation in canonical histones (fold change = 2.12, 2-sided t-test p = 0.046) and signi
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