Towards honey authentication: assessing European honey entomological origin by a molecular identification approach

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Assuring the integrity of the food chain:

Turning science into solutions

PROGRAM & BOOK OF ABSTRACTS

FOODINTEGRITY 2017 CONFERENCE

10 -11 MAY 2017

STARHOTEL DU PARC PARMA, ITALY

EDITORS

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Assuring the integrity of the food chain:

Turning science into solutions

PROGRAM & BOOK OF ABSTRACTS

FOODINTEGRITY 2017 CONFERENCE

10 -11 MAY 2017

STARHOTEL DU PARC PARMA, ITALY

EDITORS

MICHELE SUMAN - ELENA MAESTRI - PAUL BRERETON

ORGANIZED BY

&

Ensuring the Integrity of the European food chain (FoodIntegrity)

The project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement No. 613688.

www.foodintegrity2017-parma.eu

https://www.facebook.com/foodintegrity2017parma/

#FoodIntegrityParma17

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Published by Barilla, Università di Parma, Siteia Parma.

Edited by Michele Suman, Elena Maestri and Paul Brereton.

Editorial realization by Sale in Zucca.

© 2017 by Michele Suman, Elena Maestri and Paul Brereton.

(Authors of the single lecture/poster abstracts here published

are jointly-responsible for their correspondent submitted content.)

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SCIENTIFIC COMMITTEE

4 Foodintegrity 2017 Conference - Parma, Italy 10 -11 May 2017

SCIENTIFIC COMMITTEE

MICHELE SUMAN – chair Barilla SpA

ELENA MAESTRI – vice chair University of Parma, Siteia.Parma

PIER LUIGI ACUTIS Istituto Zooprofilattico Sperimentale Piemonte

MARCO ARLORIO Università del Piemonte Orientale

PAUL BRERETON Fera Science Ltd

FEDERICA CAMIN Fondazione Edmund Mach

CHRIS ELLIOTT Queen’s University Belfast

LYNN FREWER Newcastle University

DIEGO LUIS GARCIA GONZALEZ Consejo Superior de Investigaciones Científicas Sevilla

ANA GARRIDO Universidad Nacional de Córdoba

IAN GOODALL The Scotch Whisky Research Institute

JANA HAJSLOVA University of Chemistry and Technology Prague

GERALD HERRMANN Organic Services GmbH

ROLANDO LORENZETTI Consorzio Italbiotec

NIELS LUCAS LUIJCKX TNO Innovation for Life

WILL MEISTER Organic Services GmbH

VAHID MOJTAHED Fera Science Ltd

JEAN-FRANÇOIS MORIN Eurofins Analytics France SAS

PETTER OLSEN Nofima AS Tromsoe

DOLORES PÉREZ MARÍN Universidad Nacional de Córdoba

SASKIA VAN RUTH Rikilt Wageningen UR

MONIKA TOMANIOVA University of Chemistry and Technology Prague

ANDREW WATSON Institute of Food Research UK

DANIEL WUNDERLIN Universidad Nacional de Córdoba

LOCAL COMMITTEE

MICHELE SUMAN – chair Barilla SpA

ELENA MAESTRI – vice chair University of Parma, Siteia.Parma

DANTE CATELLANI Barilla SpA

DANIELE CAVANNA Barilla SpA

FRANCESCA LAMBERTINI Barilla SpA

MARZIA MANARA Barilla SpA

BARBARA PICCININI Barilla SpA

BARBARA PRANDI University of Parma, Siteia.Parma

STEFANO SFORZA University of Parma, Siteia.Parma

SANDRO ZANARDI Barilla SpA

ORGANIZING COMMITTEE

PAUL BRERETON Fera Science Ltd

MICHELE SUMAN Barilla SpA

ELENA MAESTRI University of Parma, Siteia.Parma

MONIKA TOMANIOVA University of Chemistry and Technology Prague

VERDA FAZLIC Fera Science Ltd

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CONTENT

33

Foodintegrity 2017 Conference - Parma, Italy 10 -11 May 2017 P53 CYCLIC FATTY ACIDS AS QUALITY MARKERS IN MEAT

V. Lolli, E. Zanardi, A. Marseglia, G. Palla, A. Caligiani

P54 ESTIMATION OF HEALTH RISK ASSOCIATED WITH FISH CONSUMPTION AT DIFFERENT AGE’S INDIVIDUALS

Ö. Özden, N. Erkan, M. Kaplan

P55 THE SIGNIFICANCE AND HEALTH IMPLICATIONS OF ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF BACTERIA ISOLATED FROM LOCALLY MADE SNACKS IN NIGERIA Y. Ajayi, L. Ilenikhena, O. Ayeni, G. Buraimoh Oluwatosin, A. Bello

P56 USE OF MOLECULAR ANALYSIS TECHNIQUES FOR MISLABELLING

DETECTION IN SEAFOOD PRODUCTS, COMMERCIALIZED WITH THE TERM “GALEOS” A. Imsiridou, S. Maradidou, D. Loukovitis, G. Minos

P57 GREEN TEA: AUTHENTICATION OF GEOGRAPHICAL ORIGIN BASED ON UHPLC-HRMS FINGERPRINTS

V. Hrbek, F. Kratky, K. Navratilova, M. Tomaniova, J. Hajslova

P58 TOWARDS HONEY AUTHENTICATION: ASSESSING EUROPEAN HONEY ENTOMOLOGICAL ORIGIN BY A MOLECULAR IDENTIFICATION APPROACH S. Soares, L. Grazina, I. Mafra, J. Costa, M.B.P.P. Oliveira, J.S. Amaral, M.A. Pinto

P59 SMALL GRAIN CEREAL CHAINS: TRACKING DNA TO DEFEND AUTHENTICITY AND SAFETY V. Avossa, C. Morcia, R. Ghizzoni, G. Tumino, V. Terzi, S. Scaramagli, P. Carnevali, B.E. Rahamani P60 SESAME SEED LABELLING AND HEALTH PROTECTION OF ALLERGIC CONSUMERS:

A LABORATORY SURVEY IN NORTHERN ITALY

D.M. Bianchi, L. Decastelli, C. Ippolito, S. Fragassi, S. Gallina, A. Bellio, D. Nucera P61 WHAT’S COOKING? FIRST APPROACHES OF SPECIES IDENTIFICATION

IN COMPLEX FOOD MATRICES

F. Cerutti, M.V. Riina, C. Beltramo, P.L. Acutis, S. Peletto P62 SELECTION OF DNA MARKERS FOR THE IDENTIFICATION OF

ANACARDIACEAE FAMILY MEMBERS (ANACARDIUM OCCIDENTALE AND PISTACIA VERA) J. Costa, I. Silva, C. Mendes, C. Villa, M.B.P.P. Oilveira, I. Mafra

P63 DETECTION OF MILK AND CHEESE FRAUD BY ELISA METHODS N. Sajic, L. Streppel, K. Doorn, M. Oplatowska-Stachowiak, W. Haasnoot P64 PURSUING AUTHENTICITY AND VALORIZATION

OF MEDITERRANEAN TRADITIONAL PRODUCTS, REALMED PROJECT

C. Máguas, N. Ogrinc, F. Taous, J.L. Araus, P. Reis, F. Camin, M. Lauteri, I. Novak, B. Butinar, N. Atti P65 DETECTION OF GELATIN ADULTERATION USING BIO-INFORMATICS,

PROTEOMICS AND HIGH-RESOLUTION MASS SPECTROMETRY F. Beaudry, M. Godula, C. Yang, D. Ghosh

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POSTER SESSIONS

186 Foodintegrity 2017 Conference - Parma, Italy 10 -11 May 2017

P58

TOWARDS HONEY AUTHENTICATION:

ASSESSING EUROPEAN HONEY

ENTOMOLOGICAL ORIGIN BY A

MOLECULAR IDENTIFICATION APPROACH

Keywords: honey, authenticity, western honey bee, Apis mellifera subspecies.

Following the European Union (EU) legislation, honey should be produced by the western honey bee, Apis mellifera. Across Europe, 10 different A. mellifera subspecies can be found, comprising 3 different lineages (A, M and C) based on mtDNA [1]. In general, honey bees occupy allopatric geographical ranges according to their evolutionary lineages, allowing to establish an entomological origin for honey produced in different EU countries. Additionally, several honeys with protected designation of origin (PDO) detail the subspecies traditionally used in their production [2]. While numerous works focused on the botanical and/or geographical authenticity of honey, only a few have attempted its entomological authentication. For that purpose, DNA-based methods have been considered as the most suitable tools since they allow the unequivocal species identification. So far, only few works described the use of DNA-based methods to establish the entomological origin of honey [3,4] and those were focused on different species of honey bees, including Meliponini and/or Trigonini stingless bees. To our knowledge, this is the first attempt to distinguish among different European honey bee subspecies commonly used in honey production, with further application to honey authentication.

In this work, DNA markers were developed for the differentiation of A. mellifera subspecies DNA in honey. For this purpose, individuals of A. m. iberiensis lineage A (n=22) from Portugal and Spain (n=5), A. m. iberiensis lineage M from Spain (n=7), A. m. mellifera lineage M from France, Netherlands, Scotland and Norway (n=7), A. m. ligustica lineage C from Italy (n=4), A. m. carnica lineage C from Croacia and Serbia (n=4) and commercial Buckfast lineage C bees (n=10) were tested. Different sets of primers were designed targeting the cytochrome oxidase I gene. The specificity and sensitivity of the designed primers were assayed by qualitative polymerase chain reaction (PCR). Species-specific primers successfully allowed the identification of A. m. iberiensis lineage A by end-point PCR. The use of real-time PCR coupled with High Resolution Melting analysis allowed the separation of A. mellifera honey bee subspecies in

SÓNIA SOARES, LILIANA GRAZINA, ISABEL MAFRA, JOANA COSTA,

M. BEATRIZ P.P. OLIVEIRA AND JOANA S. AMARAL

REQUIMTE-LAQV, Faculdade de Farmácia, Universidade do Porto, Portugal

M. ALICE PINTO

Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Portugal

JOANA S. AMARAL

ESTiG, Instituto Politécnico de Bragança, Portugal

[email protected]

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POSTER SESSIONS

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Foodintegrity 2017 Conference - Parma, Italy 10 -11 May 2017

different clusters according to their lineages. The developed methodologies were applied to the analysis of authentic honey samples from Portugal (produced by A. m. iberiensis lineage A), Spain (produced by A. m. iberiensis lineage M), and Italy (produced by A. m. ligustica lineage C), allowing its successful entomological origin identification.

Acknowledgements: This work has been supported by FCT (Fundação para a Ciência e Tecnologia)

through project UID/QUI/50006/2013 – POCI/01/0145/FEDER/007265 with financial support from FCT/ MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 and by the project NORTE-01-0145-FEDER-000011. S. Soares and J. Costa are grateful to FCT grants (SFRH/ BPD/102404/2014 and SFRH/BD/75091/2010) financed by POPH-QREN (subsidized by FSE and MCTES).

References

[1] Pinto, M.A., Henriques, D., Neto, M., Guedes, H. et al. Apidologie 2013, 44, 430-439.

[2] European Commission. 2017. Agriculture and Rural Development DOOR. Available from: http:// ec.europa.eu/agriculture/quality/door/list.html. Accessed March 2017.

[3] Prosser, S.W.J., Hebert, P.N. 2016. Food Chemistry, 214, 183-191.