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Methylation of the Sox9 and Oct4 promoters and its correlation with gene expression during testicular development in the laboratory mouse

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Academic year: 2019

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Figure 3 - Methylation mapping of 6 CpGs (1-145 bp) in 5’UTR region of Oct4 gene in testicular tissues at different stages of mouse development (11.5 dpc, 18.5 dpc, 5 dpp, adult testis, adult ovary and adult kidney)
Figure 4 - Expression pattern of Sox9 (A) and Oct4 (B) transcript in the testis at different developmental stages and in adult ovary and adult kidney (in Oct 4 only) through Real-time PCR (DCt for Sox9 and Oct4 was  calcu-lated for each stage in all tissue
Figure 5 - Percentage input and fold enrichment done by ChIP-qPCR to assess the H3K4me3 and H3K9me3 occupancy of Sox9 gene in (V a) & (V b) adult testis & (V c) & (V d) adult ovary

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