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Infectious Bronchitis Virus: Detection and Vaccine Strain Differentiation by Semi-nested RT-PCR

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Academic year: 2019

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Table 1 - Oligonucleotides used in Semi-N-RT-PCR.
Figure 2 shows that a fragment with the expected size (451 bp) was amplified when all four IBV strains were tested (H120, M41, Arkansas and A034).
Figure 4 - Specificity analysis of RT-PCR, using universal oligonucleotides (SYU+ and VBIS-)

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