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PLK1 and β-TrCP-dependent ubiquitination and degradation of Rap1GAP controls cell proliferation.

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Academic year: 2017

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Figure 2. Rap1GAP is ubiquitinated and degraded in a b-TrCP dependent manner. (A) 293T cells were co-transfected with Flag-Rap1GAP and the indicated Myc-tagged E3 ligases constructs (b-TrCP1, SKP2, Cdc20, Cdh1 and Fbw5)
Figure 3. PLK1 interacts with Rap1GAP and phosphorylates it at Ser525. (A) 293T cells were co-transfected with Flag-Rap1GAP and Myc- Myc-PLK1 constructs

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