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Phage-Based Fluorescent Biosensor Prototypes to Specifically Detect Enteric Bacteria Such as E. coli and Salmonella enterica Typhimurium.

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Fig 1. Characterisation of phagosensor constructs. (A) Three PCR fragments containing the gfpmut2 gene under the control of either PrrnB, PrplU or PbolA promoter (red boxes), the kanamycin  resistance-cassette and 80 bp homologous to the insertion region (
Fig 2. Determination of optimal detection conditions. (A) Bacterial survival analyzed by flow cytometry as a function of time under several temperature conditions
Fig 3. Determination of minimal detectable cell count of infected E . coli TD2158. Serial 2-fold dilutions from 10 3 to 2 bacteria.ml -1 are numbered from 1 to 10
Fig 4. Flow cytometric analysis of spiked sea water sample. E. coli TD2158 in stationary phase was added to a sea water sample to a final cell count of 2x10 6 bacteria.ml -1 , then the picked sea water sample was incubated overnight at 20°C
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