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Protein kinase A binds and activates heat shock factor 1.

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Academic year: 2017

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Figure 1. HSF1 binds to PKAc a in cells. A) Immunoprecipitation assays. HeLa cells were incubated for 1 hour at 37 u C or 43 u C before they were harvested on ice and equalized for protein concentration
Figure 2. HSF1 is phosphorylated on serine 320 by PKAc a . A) Purified GST-tagged HSF1 was phosphorylated in vitro by PKAca
Figure 4. Phosphorylation at S320 influences the intracellular localization and activity of HSF1
Figure 5. PKAc a knockdown inhibits HSF1 association with the hsp70.1 promoter. (A) Diagram of primers mapping to the hsp70.1 gene

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