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Efficient whole-cell biocatalyst for acetoin production with NAD+ regeneration system through homologous co-expression of 2,3-butanediol dehydrogenase and NADH oxidase in engineered Bacillus subtilis.

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Academic year: 2017

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Table 1. Bacterial strains, plasmids and primers used.
Table 2. Enzyme activity and the whole-cell biocatalytic activity of different strains.
Figure 5. Effect of temperature on the conversion rate of whole-cell biocatalyst. (A) Enzyme activities under different  temper-ature; (B) whole-cell biocatalyst conversion rate.
Figure 8. Time course of batch and fed-batch bioconversion of acetoin from 2,3-butanediol
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