Checklist of Ephemeroptera (Insecta) from São Mateus River Basin, Espírito Santo, Brazil

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REVISTA

BRASILEIRA

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Entomologia

AJournalonInsectDiversityandEvolution

Systematics,

Morphology

and

Biogeography

Checklist

of

Ephemeroptera

(Insecta)

from

São

Mateus

River

Basin,

Espírito

Santo,

Brazil

Kamila

Batista

Angeli

a,∗

_,

_Ezinete

_Moreira

_M.

_do

_Rozário

b

_,

_Frederico

_Falcão

_Salles

b

a_Programa_de_{Pós-Graduac¸}_ão_em_{Biodiversidade}_Tropical,_Universidade_Federal_do_Espírito_Santo,_São_Mateus,_ES,_Brazil

b_Laboratório_de_Sistemática_e_Ecologia_de_Insetos,_Departamento_de_Ciências_Agrárias_e_Biológicas,_Universidade_Federal_do_Espírito_Santo,_São_Mateus,_ES,_Brazil

a

r

t

i

c

l

e

i

n

f

o

Articlehistory:

Received12February2015 Accepted12May2015 Availableonline10July2015 AssociateEditor:RodrigoS.M.Feitosa Keywords: Adultstage Aquaticinsects Diversity Neotropicalregion Mainriver

a

b

s

t

r

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InordertoexpandtheknowledgeonthecompositionofEphemeropterafromlargerivers,wepresent, herein,thefirstsurveyofmayfliesfromtheSãoMateusRiverBasin,EspíritoSantoState.Adultswere collectedbiannuallyin2012and2013withtheaidofPennsylvanialighttrapinelevenpointsdistributed inthemainriveroftheriverbasin,SãoMateusRiveranditstwomaintributaries,CotaxéRiver(Braço Norte)andCricaréRiver(BraçoSul).Thirty-threespecieswereidentified(22nominaland11 morphos-pecies)in24generaandfivefamilies.Onegenusandonespeciesarereportedforthefirsttimefrom Brazil,threespeciesfromSoutheasternRegionofBrazil,andtwospeciesfromthestate.Fivespecies andonegenusnotpreviouslydescribedwerealsofound.Moreover,around20%oftheknownspecies ofmayflyregisteredfromthestatewerefound.Thisworkreinforcestheneedtogivemoreattentionto researchonlargeriversduetothehighpotentialfordiversity,notonlyforEphemeroptera,butalsofor otheraquaticinsects.

Introduction

Theknowledgeconcerningmayﬂies(Ephemeroptera:Insecta)

in Brazil has increased considerably in recent years. With the

increaseoftaxonomicpapersand publishedgeographical

distri-bution,thenumberofspeciesreportedfromthecountryalmost

doubledintwodecades(Da-SilvaandSalles,2012).Notlongagothe

stateofEspíritoSantowasconsideredunderstudiedwithrespect

totheorderinthecountry.However,afterthepublicationofthe

ﬁrstsurveyofEphemeroptera(Sallesetal.,2010),andaseriesof

taxonomicpapersbasedonmaterialfromthestate(Salles,2010;

De-Souzaetal.,2011;MassariolandSalles,2011;SallesandLima,

2011),thissituationhasdrasticallychanged.Thenumberofmayﬂy

speciesreportedfromthestatein2006waseight,andnowmore

than80specieshavebeenrecordedand/ordescribed.

Nevertheless,asinmoststudiesconductedinBrazil,thevast

majorityoftheserecordswereachievedbasedonmaterialcollected

instreams.MediumtolargeriversinBrazil,asageneralrule,are

neglectedwhenitcomestothestudyoftheaquaticinsects

commu-nity.Thissituationnotonlyhampersanyattemptstouncoverthe

∗ Correspondingauthor.

E-mail:[email protected](K.B.Angeli).

diversityoftheaquaticbiota,butalsopreventsdirectlyapplicable

studies,suchasbiomonitoringforexample.

AmongthemainriversinthestateofEspíritoSanto,theSão

MateusRiverislocatedinthenorthofthestateandhasa

max-imumwidthofapproximately310meters(SEAMA/IEMA,2015).

TheSãoMateusRiverBasinispartoftheEasternAtlantic

Hydro-graphicRegion(MMA,2003)andiscompletelyinsertedintothe

AquaticEcoregionoftheAtlanticForest(MMA,2006).Itisthemain

watersupplytoseveraltownsandcities,andhasbeenproviding

watertoseveralirrigationprojects,andhasalsobeenusedas

recip-ientofdomesticandindustrialefﬂuentsandtheseotherplaces.The

remainingforeststhatprotectsoilandwaterresourceshavebeen

cutorburnedovertheentirebasin(ANA,2015).

Aiming toexpand the knowledgeonmayﬂies, as wellas to

increasetheinformationaboutthisgroupinthestateofEspírito

Santo,theaimofthisworkistoprovidetheﬁrstinventoryofspecies

ofEphemeropteraintheSãoMateusRiverBasin.

Materialandmethods

Studyarea

ThestateofEspíritoSantoislocatedintheSoutheastRegion

ofBrazil;ithasabout46,000km2_and_consists_of₇₈_{municipalities}

and12RiverBasin(Feitozaetal.,2001).TheSãoMateusRiverBasin

http://dx.doi.org/10.1016/j.rbe.2015.06.004

0085-5626/© 2015 Sociedade Brasileira de Entomologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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MG 1 2 3 6 4 5 ES 0 20 Kilometers 7 8 10 E W N S 9 11

Fig.1. SamplingpointsintheSãoMateusRiverBasin,stateofEspíritoSanto.

(Fig.1)hasanareaofapproximately13,482km2_,_with₅₈₀₆_km2_in

thestateofMinasGerais,covering12municipalitiesand7676km2

inthestateofEspíritoSanto,whichpassesthrough11

municipali-ties(SEAMA/IEMA,2015).

TheSãoMateusRiverisformedbytwomaintributaries:the

CotaxéandCricarérivers,with244kmand188kmlength,

respec-tively.(SEAMA/IEMA,2015).FromthespringheadsinstateofMinas

GeraistothetownsofNovaVenéciaandBoaEsperanc¸a,boththese

watercoursesasitstributarieshavemanyrapidsandsmall

water-falls.ThelowercourseoftheSãoMateusRiverconsistsmainlyof

ﬂoodedpiemontesandasmallstripofcoastalplain,havingsoft

andwavyrelief,whichinadditiontoeasyaccessallowstheuseof

motomechanizationinmanystretches.Probably,thesearesome

reasonsfor indiscriminate process of deforestation in this area

alongthelastﬁftyyears.Todaytheentireregionisalmostdevoid

ofnativevegetationandfeaturesmanyerosionalareas.Moreover,

theriverisalsoreceptorofdomesticandindustrialefﬂuentsfrom

severalcitiesthroughwhichitpasses(ANA,2015).

Collection,identiﬁcationanddeposition

SampleswerecollectedatthemainriverofSãoMateusRiver

Basin,in11sitesdistributedasfollows:threeintheCricaréRiver,

threeintheCotaxéRiver,fourintheSãoMateusRiverandone

intheMariricuRiver(Fig.1).Sampleswerecollectedinfour

cam-paigns,between2012and2013.Figs.2–5correspondtoimagesof

someofthecollectionpoints.InTable1thesepointsarepresented

withtheirrespectivegeographiccoordinates.Sampleswere

gath-eredthroughaPennsylvanialighttrap(Frost,1957),equippedwith

a12Voltsinspectionlampandaplasticcontainercompletelyﬁlled

withalcohol92.6%.Thetrapswereplacedintreesalongthe

river-bank,andthelampwaslitnolaterthanﬁveo’clock,stayingonuntil

dawnofthenextday.

Fortheidentiﬁcationofthematerial,aseriesofbooks,theses

andpapershave beenconsulted,amongthem:Molineri(1999),

Domínguezetal.(2006),Salles(2006),Molineri(2010),Gonc¸alves etal.(2011),Nascimentoetal.(2011),Limaetal.(2012),Cruzetal. (2014).ThespecimensaredepositedatColec¸ãoZoológicaNorte

Capixaba(CZNC)ofUniversidadeFederaldoEspíritoSanto,stored

ataround−20◦_C_in_80%_ethanol.

Materialexaminedandgeographicaldistribution

The geographical distribution presented for the taxa was

extractedfromDomínguezetal.(2006)andSallesetal.(2015).

TheexaminedmaterialisorganizedsothatPTreferstothe

collec-tionpoint(Table1),inbracketsisthenumberofindividuals,and

thenthedateofcollection.Newrecordsofthespeciesaremarked

withanasterisk(*).

Results

Altogether33speciesarelisted,with23nominaland10

mor-phospecies,24generaandﬁvefamilies.Figures6–9representsome

ofthecollectedspecies.Thespeciesandmorphospeciesfoundin

theSãoMateusRiverBasinarelistedbelow.

Foreach species,theirgeographical distributionispresented

aswellasthepointsatwhichitwascollected(moreinformation

aboutthecollectionpointsarepresentedinTable1).Commentsare

addressedwhenpertinent.

Baetidae

AmericabaetisalphusLugo-OrtizandMcCafferty,1996

Geographical distribution: Argentina, Bolívia, Chile, Paraguai

andBrazil:Alagoas,Amazonas,Bahia,Ceará,EspíritoSanto(Alto

Caparaó,Ibitirama,SantaTeresa,SãoMateus*andNovaVenécia*),

Goiás,MatoGrosso,MinasGerais,Paraná,Pernambuco,Piauí,Rio

deJaneiro,RioGrandedoSul,Roraima,SantaCatarina,SãoPaulo

andSergipe.

Comments:PreviouslyrecordedforthestateofEspíritoSanto

(Sallesetal.,2010),butfortheﬁrsttimeinthemunicipalitiesof

SãoMateusandNovaVenécia.

Material examined: PT01: (1) 16–17/iv/2012; PT01: (1)

20–21/xi/2012;PT02:(1)23–24/v/2012;PT02:(1)22–23/xi/2012;

PT03: (1) 26–27/vii/2012; PT03: (1) 20–21/xi/2012; PT04: (1)

21–22/XI/2012;PT05:(1)21–22/xi/2012.

AturbinabeatrixaeGillies,2001

Geographicaldistribution:Argentina,UruguaiandBrazil:Bahia,

Espírito Santo (Município Alto Caparaó, São Mateus), Mato

Grosso, Minas Gerais, Paraná, Pernambuco, SantaCatarina, São

Paulo.

(Sallesetal.,2010),butfortheﬁrsttimeinthemunicipalityofNova Venécia.

Material examined: PT03: (2) 20–21/xi/2012; PT04: (10)

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Figs.2–5.Someofcollectionpoints.2.CotaxéRiver,PatrimôniodoBis.3.CricaréRiver,FazendaLiberdade.4.SãoMateusRiver,SítioSantaMaria.5.SãoMateusRiver,Guriri.

Table1

LocalitiessampledintheSãoMateusRiverandtheircoordinates.

Points Municipality Locality Coordinates

PT01 NovaVenécia PatrimôniodoBis,RioCotaxé S18◦3327.5,W40◦206.5

PT02 SãoMateus CachoeiraJapira,RioCotaxé S18◦₃₄_26.6_,_W40◦₁₆_35.27

PT03 SãoMateus PropriedadedoSr.FábioGama,RioCotaxé S18◦₃₇_41.1_,_W₄₀◦₆_41.6

PT04 NovaVenécia SantaRitadoPipNuck,PropriedadedoSr.Puppim,RioCricaré S18◦₃₉_51.4_,_W₄₀◦₃₀_44.9

PT05 NovaVenécia CasadaDonaAna,RioCricaré S18◦₄₂_54.91_,_W₄₀◦₂₂_33.33

PT06 SãoMateus FazendaLiberdade,RioCricaré S18◦₃₉_2.2_,_W₄₀◦₇_23.4

PT07 SãoMateus SítioSantaMaria,RioSãoMateus S18◦3917.1,W39◦5936

PT08 SãoMateus BairroJambeiro,RioSãoMateus S18◦4140.3,W39◦5151.5

PT09 SãoMateus BairroPedrad’água,RioSãoMateus S18◦₄₃_15.2_,_W39◦₄₈_50.8

PT10 SãoMateus EstradaparaBarraNova,RioMariricu S18◦₄₇₁₁_,_W39◦₄₇_31.6

PT11 SãoMateus Meleiras,RioSãoMateus S18◦₃₉_39.5_,_W39◦₄₅₄₈

CallibaetisguttatusNavás,1915

Geographical distribution: Argentina and Brazil: Ceará,

Pernambuco,RiodeJaneiroeEspíritoSanto(SãoMateuseNova

Venécia*).

(Sallesetal.,2010),butfortheﬁrsttimeinthemunicipalityofNova Venécia.

23–24/v/2012.

Camelobaetidiussp.

Occurrenceinthestudiedarea:EspíritoSanto,municipalityof

SãoMateus.

Comments: Identiﬁcation at species level was not possible,

becauseonly one adultwas analyzed and theassociation with

nymphsisessentialfortherecognitionofthespecies.

Materialexamined:PT02:(1)23–24/v/2012.

Paracloeodescf.waimiri

NovaVenécia.

Comments:ProbablyitisParacloeodeswaimiriNietoandSalles

basedonabdominalcolorpattern;howeveritisnecessary

associ-ationwithnymphsforconﬁrmation,sinceonlyadultindividuals

wereanalyzed.

18–19/iv/2012.

Paracloeodessp.1

NovaVenécia.

becauseonly oneadultwasanalyzed, and theassociationwith

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Figs.6–9.AdultsofEphemeroptera.6.Ulmeritoidessp.7.Traverellainsolita.8.Aturbinabeatrixae.9.Hydrosmilodonplagatus.

22–23/xi/2012;PT03:(4)20–21/xi/2012;PT04:(1)18–19/iv/2012;

PT04:(215)21–22/xi/2012;PT05:(155)21–22/xi/2012.

Caenidae

Caenissp.nov.

NovaVenéciaandSãoMateus.

Comments:ThisspeciesisbeingdescribedbyLimaetal.(Pers.

Comm.,2015).

21–22/xi/2012;PT04:(1)18–19/ii/2013;PT05:(1)23–24/v/2012;

PT05:(4)26–27/vii/2012;PT07:(1)07–08/ix/2012.

AlloretochussigillatusMolineri,2014

Geographicaldistribution:Bolivia,EquatorandBrazil*:Espírito

Santo(NovaVenécia).

Comments:FirstrecordofthegenusandspeciesfromBrazil.

20–21/xi/2012; PT02:(6)22–23/xi/2012;PT03:(112) 20–21/xi/

2012; PT03: (80) 05–6/ii/2013; PT04: (19) 18–19/iv/2012;

PT04: (22) 21–22/xi/2012; PT04: (2) 18–19/ii/2013; PT06: (3)

22–23/xi/2012;PT06:(1)07–08/ii/2013.

Leptohyphidae

Gen.nov.sp.nov.

NovaVenécia.

Comments:Thisisanewgenusapparentlyrelatedto

Trichory-thodes.Aspeciesbelongingtothesamegenushasalsobeenfound

inSerradoAracá,AmazonasState.

Materialexamined:PT04:(1)25–26/vii/2012.

Macunahyphesaustralis(Banks,1913)

Geographicaldistribution:Argentina,GuianaandBrazil:Mato

Grosso,Pará,Paraná,RoraimaandEspíritoSanto*(SãoMateusand

NovaVenécia).

Comments:FirstrecordforSoutheastRegion.

23–24/v/2012;PT02:(2)07–08/ix/2012;PT04:(2)18–19/iv/2012;

PT05: (2) 23–24/v/2012; PT07: (1) 07–08/ix/2012; PT09: (1)

19–20/iv/2012.

Traverhyphes(Mocoihyphes)yuatiMolineri,2004

Geographicaldistribution:ArgentinaandBrazil:RiodeJaneiro,

SãoPaulo,Maranhão,Piauí,GoiásandEspíritoSanto(SãoMateus*

andNovaVenécia*).

Comments: Previously registered for state of Espírito Santo

(Sallesetal.,2010),butfortheﬁrsttimeinmunicipalitiesofSão

MateusandNovaVenécia.

Material examined: PT01: (444) 16–17/iv/2012; PT01:

(11) 25–26/vii/2012; PT02: (70) 23–24/v/2012; PT01: (57)

21–22/xi/2012;PT01 (224)05–06/ii/2013; PT02:(14)07–08/ix/

2012; PT03: (331) 16–17/iv/2012; PT03: (212) 26–27/vii/2012; PT03:(1308)20–21/xi/2012; PT03:(2392)05–06/ii/2013;PT04: (1449) 18–19/iv/2012; PT04: (787) 25–26/vii/2012; PT04: (2276) 21–22/xi/2012; PT04: (213) 18–19/ii/2013; PT04: (484) 15–16/iv/2013;PT05:(110)21–22/xi/2012;PT05:(15)23–24/v/ 2012; PT05: (1) 26–27/vii/2012; PT06: (945) 22–23/v/2012;

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PT06: (102) 08–09/ix/2012; PT06: (114) 22–23–06/xi/2012;

PT06: (682) 05–06/ii/2013; PT06: (315) 07–08/ii/2013; PT07:

(826) 07–08/ix/2012; PT07: (250)13–14/vi/2012; PT07:(1984)

05–06/ii/2013;PT08:(4)22–23/v/2012.

Traverhyphes(Traverhyphes)piraiMolineri,2001

Geographicaldistribution:Brazil:RiodeJaneiro,Pernambuco

andEspíritoSanto*(SãoMateusandNovaVenécia).

Comments:FirstrecordofstateofEspíritoSanto.

20–21/xi/2012; PT02: (170) 06–07/ii/2013; PT03:(2) 20–21/xi/

2012PT04: (143) 18–19/iv/2012; PT04: (17) 25–26/vii/2012;

PT04: (31) 21–22/xi/2012; PT04: (11) 18–19/ii/2013; PT05:

(3)23–24/v/2012; PT05: (5) 21–22/xi/2012; PT06: (1) 08–09/

ix/2012;PT06:(1)07–08/ii/2013;PT07:(1)13–14/vi/2012;PT07:

(2)05–06/ii/2013.

TricorythodesmircaMolineri,2002

Geographicaldistribution:BoliviaandBrazil:Maranhão,Piauí,

PernambucoandEspíritoSanto(SãoMateus,SooretamaandNova

Venécia*).

Comments:PreviouslyregisteredforthestateofEspíritoSanto

(Sallesetal.,2010),butﬁrstrecordedforthemunicipalityofNova Venécia.

25–26/vii/2012;PT01:(6)20–21/xi/2012;PT03:(1)06–07/ii/2013;

PT03: (7) 20–21/xi/2012; PT03: (3) 05–06/ii/2013; PT04: (2) 18–19/iv/2012; PT04: (1) 25–26/vii/2012; PT04: (17) 21–22/xi/2012;PT05:(5)23–24/v/2012;PT05:(1)26–27/vii/2012; PT05: (10) 21–22/xi/2012; PT05: (4) 18–19/ii/2013; PT06: (1) 22–23/v/2012; PT06: (1) 08–09/ix/2012; PT06: (26) 22–23/xi/2012;PT07:(5)07–08/ix/2012;PT08:(1)10–11/v/2012; PT09:(5)19–20/iv/2012;PT10:(1)04–05/ii/2013.

TricorythodessantaritaTraver,1959

Geographical distribution: Brazil: Rio de Janeiro e Espírito

Santo*,municipalitiesofNovaVenéciaandSãoMateus.

Comments:FirstrecordforthestateofEspíritoSanto.

23–24/v/2012;PT03:(1)26–27/vii/2012;PT04:(5)18–19/iv/2012;

PT 04:(2) 25–26/vii/2012; PT05: (17)23–24/v/2012;PT06: (1)

08–09/ix/2012;PT06:(1)07–08/ii/2013;PT07:(2)13–14/vi/2012;

PT07:(3)07–08/ix/2012.

TricorythopsisartigasTraver,1958

Geographicaldistribution:Argentina, Uruguaiand Brazil:Rio

GrandedoSul,RiodeJaneiroandEspíritoSanto(AlfredoChaves,

SantaTeresa,SãoMateus*andNovaVenécia*).

(Salleset al.,2010),but ﬁrstregisteredin municipalitiesofSão

Material examined: PT01: (2) 25–26/vii/2012; PT01: (159)

25–26/vii/2012;PT02:(4)23–24/v/2012;PT02:(2)06–07/ii/2013;

PT04:(19)25–26/vii/2012.

Tricorythopsisminimus(Allen,1973)

Geographical distribution: Argentina, Uruguai, Brazil: Rio

GrandedoSulandEspíritoSanto(Sooretama,SãoMateus*andNova

Venécia*).

(Salleset al.,2010),but ﬁrstregisteredin municipalitiesofSão

Material examined: PT01: (4) 16–17/v/2012; PT01: (809)

25–26/vii/2012; PT02: (43) 23–24/v/2012; PT03: (1) 20–21/

xi/2012; PT03: (2) 05–06/ii/2013; PT04: (14) 25–26/vii/2012;

PT05: (54) 23–24/v/2012; PT05: (1) 26–27/vii/2012; PT05: (4)

21–22/xi/2012;PT07:(1)07–08/ix/2012;PT07:(16)05–06/ii/2013.

TricorythopsisyacutingaMolineri,2001

Geographicaldistribution:ArgentinaandBrazil:RioGrandedo

SulandEspíritoSanto*(SãoMateusandNovaVenécia).

Comments:FirstrecordedfortheSoutheastRegion.

(102) 25–26/vii/2012; PT02: (50) 23–24/v/2012; PT03: (1)

26–27/vii/2012; PT03: (1) 20–21/xi/2012; PT04: (4) 18–19/iv/

2012; PT04: (77) 25–26/vii/2012; PT04: (2) 21–22/xi/2012;

PT05: (2) 23–24/v/2012; PT05: (1) 21–22/xi/2012; PT07: (1)

13–14/vi/2012. Leptophlebiidae

FarrodescariocaDomínguez,MolineriandPeters,1996

Geographical distribution:Brazil:Rio deJaneiro andEspírito

Santo(Ibitirama,SantaTeresa,Sooretama,SãoMateus*andNova

Venécia*).

(Salleset al.,2010), butﬁrstregisteredin municipalitiesofSão

05–06/ii/2013;PT02:(2)07–08/ix/2012;PT03:(1)26–27/vii/2012;

PT04:(23)18–19/iv/2012;PT04:(102)25–26/vii/2012;PT04:(2)

18–19/ii/2013;PT06:(1)08–09/2012;PT07:(1)07–08/ix/2012.

HylisterobliquusNascimentoandSalles,2013

Geographicaldistribution:Brazil:EspíritoSanto(Pinheiros,São

Mateus*andNovaVenécia*).

Comments: Species recently described and recorded for the

stateofEspíritoSanto(NascimentoandSalles,2013),butforthe

ﬁrsttimeinmunicipalitiesofSãoMateusandNovaVenécia.

(2) 25–26/vii/2012; PT01: (53) 20–21/xi/2012; PT01: (2)

05–06/ii/2013;PT02:(2)07–08/ix/2012;PT04:(6)18–19/iv/2012;

PT04: (6) 25–26/vii/2012; PT04: (8) 20–21/xi/2012; PT04: (5)

15–16/iv/2013.

HermanellanigraNascimentoandSalles,2013

Geographicaldistribution:Brazil:EspíritoSanto(NovaVenécia,

RioBananal,SãoMateus).

Comments: Species recently described and recorded for the

stateofEspíritoSanto(NascimentoandSalles,2013).

Materialexamined:P1:(3)20–21/xi/2012.

HydrosmilodonplagatusLima,NascimentoandSalles,2012

Geographical distribution: Brazil: Pernambuco and Espírito

Santo(SooretamaandNovaVenécia*).

(Limaetal.,2012),butfortheﬁrsttimeinthemunicipalityofNova

Venécia.

25–26/vii/2012; PT04: (53) 21–22/xi/2012; PT04: (3) 15–16/

iv/2013;PT04:(8)18–19/ii/2013.

Simothraulopsissp.nov.1

Comments:ThisspeciesisbeingdescribedbyNascimentoetal.

(Pers.Comm.,2015).

25–26/vii/2012; PT03: (1) 16–17/iv/2012; PT03: (1) 26–27/

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Simothraulopsissp.nov.2

NovaVenécia.

Comments:ThisspeciesisbeingdescribedbyNascimentoetal.

(Pers.Comm.,2015).

25–26/vii/2012; PT04: (34) 18–19/iv/2012; PT04: (5) 25–26/

vii/2012.

Thraulodessp.

Occurrence in the studied area: Brazil: Espírito Santo (São

Mateus*).

becausethesinglespecimenwascollectedatthesubimagostage.

FirstrecordofthegenusforthemunicipalityofSãoMateus.

Material examined: PT03: (1) 05–06/ii/2013; PT06: (1)

22–23/v/2012;PT06:(1)08–09/ix/2012.

TerpidessooretamaeBoldriniandSalles,2009

Geographical distribution: Brazil: Mato Grosso, Pernambuco

andEspíritoSanto(AlfredoChaves,BarraSeca,Sooretama,Nova

Venécia*andSãoMateus*).

(BoldriniandSalles,2009),butfortheﬁrsttimeinthe

municipali-tiesofNovaVenéciaandSãoMateus.

21–22/xi/2012.

Tikunabilineata(NeedhamandMurphy,1924)

Geographicaldistribution:Colombia,Equador,Peru,Suriname,

VenezuelaandBrazil:MatoGrosso,ParáandEspíritoSanto*(Nova

Venécia).

Comments:FirstrecordfortheSoutheasternRegion.

18–19/iv/2012.

TraverellainsolitaNascimentoandSalles,2013

Geographicaldistribution:Brazil:EspíritoSanto(PedroCanário,

Sooretama,NovaVenécia*).

Comments:FirstrecordforthemunicipalityofNovaVenécia.

(1) 25–26/vii/2012; PT01: (2) 05–06/ii/2013; PT03: (12)

16–17/iv/2012;PT03:(8)05–06/ii/2013;PT04:(1)18–19/ii/2013;

PT04: (5) 15–16/iv/2013; PT07: (1) 13–14/vi/2012; PT07: (1)

07–08/ix/2012.

Ulmeritoidessp.1

Occurrenceinthestudiedareas:EspíritoSanto,municipalityof

becauseindividualswerecollectedonlyatsubimagostage.

05–06/ii/2013;PT02:(2)23–24/v/2012;PT02:(1)07–08/ix/2012;

PT02: (1) 06–07/ii/2013; PT03: (2) 16–17/iv/2012; PT03: (59)

20–21/xi/2012;PT03:(2)05–06/ii/2013;PT04:(1)18–19/iv/2012;

PT04: (2) 18–19/ii/2013; PT05: (3) 18–19/ii/2013; PT06:

(1) 21–22/xi/2012; PT07: (2) 13–14/vi/2012; PT07: (11)

07–08/ix/2012;PT07:(3)05–06/ii/2013.

Polymitarcyidae

AsthenopodeschumucoMolineri,SallesandPeters,2015

Geographicaldistribution:Colombia,GuyanaandBrazil:

Ama-zonasandEspíritoSanto(SãoMateus).

(Molinerietal.,2015).

07–08/ix/2012;PT03:(2)05–06/ii/2013;PT07:(4)07–08/ix/2012.

CampsurustruncatusUlmer,1920

Geographical distribution: Bolívia and Brazil: Espírito Santo

(SantaTeresa,NovaVenécia*andSãoMateus*).

Comments:Registered in Brazil,only inthestateof Espírito

Santo.FirstrecordforthemunicipalitiesofSãoMateusandNova

Venécia.

21–22/xi/2012;PT06:(1)08–09/ix/2012;PT07:(4)13–14/vi/2012;

PT07:(3)07–08/ix/2012;PT10:(1)20–21/xi/2012.

Campsurussp.nov.

Occurrenceinthestudiedarea:EspíritoSanto,municipalitiesof

Comments:ThisnewspeciesisapparentlyrelatedtoCampsurus

segnisNeedhamandMurphy,1924.

(89) 25–26/vii/2012; PT01: (2) 05–06/ii/2013; PT02: (10) 23–24/v/2012; PT02: (16) 06–07/ii/2013; PT03: (16) 16–17/iv/ 2012; PT03: (9) 26–27/vii/2012; PT03: (43) 05–06/ii/2013; PT04: (139) 18–19/iv/2012; PT05: (1) 18–19/ii/2013; PT06: (16) 22–23/v/2012; PT06: (34) 08–09/ix/2012; PT07: (26) 13–14/vi/2012; PT07: (27) 07–08/ix/2012; PT07: (66) 05–06/ ii/2013.

TortopsiscanumGonc¸alves,Da-SilvaandNessimian,2011

Geographicaldistribution: Brazil:Rio deJaneiroand Espírito

Santo.

05–06/ii/2013;PT03:(1)16–17/iv/2012;PT03:(1)26–27/vii/2012;

PT03: (3) 05–06/ii/2013; PT06: (1) 22–23/v/2012; PT06: (3)

08–09/ix/2012;PT06:(4)07–08/ii/2013;PT07:(5)13–14/vi/2012;

PT07:(1)07–08/ix/2012;PT07:(30)05–06/ii/2013.

TortopusharrisiTraver,1950

Geographical distribution:Paraguai and Brazil:MatoGrosso,

MatoGrossodoSulandEspíritoSanto(SãoMateusandNova

Vené-cia).

05–06/ii/2013; PT02: (8) 05–06/ii/2013; PT02: (269) 06–07/

ii/2013; PT03: (1) 16–17/iv/2012; PT03: (300) 05–06/ii/2013;

PT04: (4) 18–19/ii/2013; PT06: (16) 22–23/v/2012; PT06: (1)

08–09/ix/2012; PT06: (140) 07–08/ii/2013; PT07: (7) 13–14/

vi/2012;PT07:(13)07–08/ix/2012;PT07:(283)05–06/ii/2013.

Discussion

ThepresentstudygivestheﬁrstrecordofAlloretochussigillatus

Molineri,2014forBrazil.Thegenusandspecieswerepreviously

recordedonlyfromBoliviaandEcuador.Threespeciesare

regis-teredfortheﬁrsttimefromtheSoutheasternregion[Macunahyphes

australis (Banks, 1913), Tricorythopsis yacutinga Molineri, 2001

andTikunabilineata(NeedhamandMurphy,1924)].Fivespecies

(M.australis,Traverhyphes(Traverhyphes)pirai,Molineri2001,Ti.

yacutinga,Tricorythodessantarita Traver,1959,Tk.bilineata)and

onegenus(TikunaSavage,FlowersandPorras,2005)arereported

fortheﬁrsttimefromthestateofEspíritoSanto.Furthermore,some

speciespreviouslyregisteredforstateofEspíritoSantohadtheir

distribution slightly expanded: Americabaetis alphus Lugo-Ortiz

(7)

2004,Tricorythopsisminimus (Allen,1973), Tricorythopsis artigas

Traver, 1958,Farrodes carioca Domínguez, Molineri and Peters,

1996,HylisterobliquusNascimentoandSalles,2013wererecorded

forSãoMateusandNovaVenécia,CallibaetisguttatusNavás1915

andHydrosmilodonplagatusLima,NascimentoandSalles,2012for

NovaVenécia.

Threeundescribedspecieswerefound,oneofCaenisStephens,

1835(Caenidae)andtwoofSimothraulopsisDemoulin,1966

(Lep-tophlebiidae),besidesoneundescribedgenusandspeciesofthe

familyLeptohyphidae.Takingintoaccountthespeciespreviously

reportedforthestate,around20%werefoundduringthisproject.

Two morphospecies of Leptophlebiidae (Thraulodes sp. and

Ulmeritoidessp.)and threeofBaetidae(Camelobaetidiussp.,

Par-acloeodescf.waimiriandParacloeodessp.)couldnotbeidentiﬁed,

becausetheirdiagnosticcharacterscannotbeseenbasedonthe

specimenscollected(Leptophlebiidae)orbecausetheassociation

withnymphsisessentialfortherecognitionofthespecies

(Baeti-dae).

Withrespecttothefamilies,Leptophlebiidaewasthemost

rep-resentativewith11 species(33.3%), followed byLeptohyphidae

withninespecies(27.2%),andBaetidaewithsixspecies(18.2%).

ThelowestnumberofspecieswasrecordedforPolymitarcyidae

and Caenidae withﬁve(15.1%) and two (6.1%) species,

respec-tively.Generally,instudiesdevelopedinSouthAmerica(orBrazil),

Baetidaeisthemostrepresentativefamily,closelyfollowedby

Lep-tophlebiidaeandthenLeptohyphidae(Sallesetal.,2004,2010a;

Francischetti,2007;Shimanoetal.,2011;Limaetal.,2012).

How-ever,thecompositionofthemayﬂyfaunawasdistinctfromthose

foundin studiesperformedinSoutheasternRegionandstateof

EspíritoSanto (e.g.Boldriniet al.,2009;SallesandNascimento,

2009;Moreiraetal.,2010;Sallesetal.,2010;Salles,2010;Lima et al., 2012; Barcelos-Silva et al., 2012).The differenceof

pro-portionoffamiliesandcompositionofspeciescanbeattributed

totwouncommonapproaches.First,wecapturedadultsinstead

of nymphs. This fact favored not only the ﬁnding of families

wherenymphshavecryptichabitats,suchasPolymitarcyidaeand

Caenidae,butalsotheidentiﬁcationatthespecieslevelofmostof

thefamiliesexceptforBaetidae(theonlygroupamongmayﬂies

inwhichtaxonomyisbasedalmostexclusivelyonnymphalstage).

Thesecondapproach,certainly,isduetothesizeand

characteris-ticsofthewaterbody.Rivers,giventheamountofﬁnesediment

andtheexistenceofsemi-lenticareas,aremuchmoresuitablefor

CaenidaeandPolymitarcyidae(Domínguezetal.,2006,2009;

Da-SilvaandSalles,2012).Thehighdiversityofspeciesandtheamount

ofnewrecordsandtaxaundescribeddemonstratetherelevanceof

largeriversinresearchesonaquaticinsectsfauna.

Conﬂictsofinterest

Theauthorsdeclarenoconﬂictsofinterest.

Acknowledgments

WethankthecollaborationofErikcsenRaimundiandMaisa

Car-valhofor theirhelpduringthecourseof thisworkandFabiana

Massariolbyhersupportandsuggestions.TheConselhoNacional

deDesenvolvimentoCientíﬁcoeTecnológico(CNPq)forﬁnancial

supporttotheproject“Caracterizac¸ãoambientaldabaciadorio

SãoMateusfrenteaaspectosnaturaiseantrópicos:escala

espa-cialetemporal”(ProcessnumberPELD558246/2009-5)andfora

productivitygrant toFFS,and toFAPES(Fundac¸ãodeAmparoa

PesquisadoEspíritoSanto)process#54689627/2011forﬁnancial

supporttotheproject“DiversidadeeTaxonomiadeEphemeroptera

(Insecta)doEspíritoSanto”;theUniversidadeFederaldoEspírito

Santo(UFES)forgrants awardedduringthework.TheInstituto

Chico Mendes de Conservac¸ão da Biodiversidade (ICMBio) and

InstitutoBrasileiro do MeioAmbienteeRecursosNaturais

Ren-ováveis(IBAMA)(Processnumber 12777-1)forthepermissions

ofcollectionstotherealizationofthisworkandallpeoplewho

allowedourentryintotheirpropertiesforconductingthisstudy.

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