Separation of human immunoglobulin G subclasses using monolith technology

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Por

2014

12th

International Chemical

and Biological Engineering

Conference

PORTa 10-12 SEPT.

PORTO

FEUP

FACUlDADE DE ENGENHARIA

UNIVERSIDADE DO PORTO

ORDEM

DOS

ENGENHEIROS

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Por

2014

IIpORTO

10 - 12 Sept. 2014

PORTO

PORTUGAL

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ORDEM

~~ENHEIROS

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International Chemical and Biological

Engineering Conference

BOOK OF

SHORT ABSTRACTS

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This volume contains the short abstracts presented at the 12th International Chemical and Biological Engineering Conference - CHEMPOR 2014, held in Porta, Portugal, between

September 10th _{and 12th, 2014.}

CHEMPOR 2014 was organized

by

FACULDADE DE ENGENHARIA DA UNIVERSIDADE DO PORTO

&

ORDEM Dos ENGENHEIROS

Title: Book of Short Abstracts of the 12th International Chemical and Biological Engineering Conference - CHEMPOR 2014

Published by:

Faculdade de Engenharla Unlversldade do Porta

http://chempor.ordemengenheiros.pt

Edited by A. Alves, N. F. Azevedo, J. M. lourelro, l. M. Madeira, V. B. Ollveira, M. F. R. Pereira, A. M. F. R. Pinto, P. C. R. Pinto, R. J. Santos, A. M. T. Silva

First edition August 2014

ISBN. 978-972-752-169-2

Dep6sito legal: 379460/14 Tiragem: 350 exemplares

FEUP Edi~oes

http://feupedicoes.fe.up.pt

Universldade do Porta, Faculdade de Engenharia, Departamenta de Engenharia Qufmica, Rua Or, Robertos Frias sin 4200 - 465 Porta, PORTUGAL

©FEUP

PrInted by: Serslllto - Empresa Gratka, Lda.

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Immobilization and Stabilization of p~galactosidase on Duolite A568 by Multipoint Attachment

P-BE52 63

L.N.S.s. Falleiros, W.G. Morais Junior, B.V. CabraI. L.D. Santos, M.M.Resende, V.L. Cardoso,E.J. Ribeiro

Granulated Industrial Cork and Cork Powder as Sources of High Added-Value Chemicals

P-BE53 63

Y.A. Manrique, M.V. Oliveira, P. Pinto, J.M. Loureiro

POSTER SESSION - CHEMICAL ENGINEERING FUNDAMENTALS AND PRACTICE

GE model and optimization metbod combinations for prediction of vapor composition

in binary vie from T-x data

P-FPl 65

A.R. Prasad, J.S. Babji, AA Kumar

A comparative study on mixing rule and alpha-function combinations for correlatloD of binary vapor liquid equiUbrium data by equation of state

P-FP2 65

AR. Prasad, J.S. Babji, A.A. Kumar

Design Of Hydrogel Membranes With Embedded Magnetic Particles

P-FP3 65

AC. Manjua, F.V. Chavez,

P.J.

Sebastia~. J.G. Crespo, C.A.M. Portugal

Separa):lon OfHtunaD Immunoglobulin G SUbclasses Uslog Monolith Technology

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P. Leblebici, M.E. Leblebici, F. Ferreira-da-Silva, AE. Ribeiro, AE. Rodrigues, L.S. Pais

Microprotect project -The use of encapsulated essential oils for protection of stored cereals and legumes

P-FP5 66

C.F.Silva, J.M.Sousa. F.D.Magalhaes, M.M.S.M,Bastos

Analysis oflnternal Variables of a Distillation Column Operating with Distributed Control

P-FP6 66

G.N. Mello. RA.F. Machado, C. Marangoni, .

Cata1ysts Screening for tbe Reaction of Nitrobenzene Hydrogenation

P-FP7 67

C. Sa Couto, L.M. Madeira, C.P. Nunes, P. Araiijo

Simultaneous Removal of C02 and VOC using a Two Phase Partitioning Reactor

P-FP8 67

A Couvert, D, G6mez-Diaz, D. L6pez-Rivas. J.M. Navaza

COz Absorption by Primary Amines: E:IJect of Chemical Structure and Loading upon

Reaction Pathway

P-FP9 67

A. Garda-Abuin, D, G6mez-Dfaz, D. Lopez-Rivas, J.M. Navaza, A. Rumba

Synthesis and Characterization of ALP04-17 and Evaluation of the Diffus Paraffins CI0-C13 by ZLC Technique.

P-FP10

DAA. Games, S.Mattedi, M.M. Urbina. AO.S.Silva, M. Bastos-Neto, L.A.M. Pant{ Vinasse clarification by a combined treatment of coagulation! flocculaU adsorption

P-FPll

F,L Seixas, D.R.A. Okada, M.B. de Abreu, M.L. Menezes, S.H.B, de Faria, N.R.C. F!

Machado, M.L. Gimenes

Selective Extraction of (Bio)molecules by Applying Novel Aqueous Micel

Systems Composed of Triton X-114 and Ionic Liquids as Co-surfactants

P-FP12

F.A. Vicente, L.P. Malpiedi, AP. Junior, J.A.P. Coutinho, S.P.M. Ventura

Adsorption of Vanillin and Syrlngaldehyde by Polymeric Resins: Equilihl adsorption studies

P-FP13

), Mota, P.C. Pinto, J.M. Loureiro, AE. Rodrigues

Influence ofionic liquids as electrolytes in polymer-polymer aqueous bi]

P-FP14

J.H. Santos, JAP. Coutinho, APessoa Jr., SP,M. Ventura

Chemometrics to Study the Ferric Sulphate Leaching of a Portuguese Sph

Concentrate

P-FP15

S.M.C. Santos, M.T.A. Reis, M,RC.lsmael, M.J.N. Correia, J.M.R Carvalho

MOnitoring a Polyesterification Reaction by in-line Near~lnfrared Spectn

P-FP16

H.I. Mota. M,I,N. Correia, J.C.M. Bordado

A Comparison Study of Single-Scale and MuJtiscale Approaches for Data-I Model-Based On-line Denois

P-FP17

R.R. Rendall, M.S, Reis

Potential use of chesbtut (Castanea sativa M_) husk ashes as a source for]

production

P-FP18

M,O. Tones,

J.

Seijo

Mechanical Properties of Natural Potassium Soaps Obtained of By-produ Chesbtut Industry

P-FP19

M.D. Torres,

J.

Seijo

Fiber flow velodty estimation using Electrical Impedance Tomography

P-FP20

P.M. Faia. B. Braneo. F. Garcia. M,G. Rasteiro

Production of chitosan microparticles cross-linked with geoipin for drug

applications: A DoE approach to identify factors influencing size and shaI

P-FP21

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Separation Of Human ImmunoglobuUn G Subclasses Using Monolith Technology

P·FP4

Pe/in Leblebici', "', M. Erlis Leblebicl. Frederico Ferreira-da-Silw/. Anlonio E. RibeiroJ

, A/Mo E. Rodrigues'. Luis

S. Pai/, (1) Laboratory a/Separation and Reaction Engineering. Associate Laboratory LSREILCM, Department of

Chemical Engineering. Faculty of Engineering, University of Porta, RUG Dr. Roberto Frias sin, 4200-465 Porto,

Portugal; ·Correspondlng author: e-mail address:pellin.arslan@gmail.com. (2) fBMe -Institute for Molecular and

Cell Biology, University a/Porto, 4150-/80 Porta, Portugal'(3) Laboratory a/Separation and Reaction Engineering,

Associate Laboratory LSRE/LeM, School of Technology and Management, PolytechniC Institute of Bragan~a,

Campus de Santa ApolOnia, Apartado 1134. 5301-857 Braganfa, Portugal

In the present study, a step gradient elution method was evaluated for the separation of human immunoglobulin G (hIgG) into its subclasses on CIM rwprotein A monolithic column. hIgG

Seheme 1. Represantative structures of the human IgO subclasses [I).

was loaded onto the column and bound protein was eluted with a pH gradient. The subclass content of the eluted fractions was analyzed by enzymewIinked immunosorbent assay (ELISA). Results showed that separation of IgG3 from the other three subclasses can be successfully achieved with high selectivity (100%) and throughput on monolithic media. It was also revealed that enriched fractions of IgG 1 and IgG2 could be obtained from purified hIgG in a 28 minutes long chromatographic run, Three fractions with high IgO 1 content (89.1%,94,3% and 88.8%) were recovered, Furthennore, IgG2 was enriched to 64% successfully. A rapid step gradient elution scheme without any additives in buffers was proven to obtain enriched prep~ions of the two important subclasses with high througbput. .'

Microprotect project w The use of encapsulated essential oils for

protection of stored cereals and legumes

P·FP5

CoF.Si/vol

.'. J.MSoU1al, F.D.Magalhtles', MMS.M.Bastosl, (1) LEPABE - Faculdade de Engenharia, Universidade

do Porto, Rua Dr. Roberto Frias, 4200-465 Porta. Portugal; 'mbastos@fe.up.pt.

Food safety is a problem that concerns society nowadays. The current preservative techniques rely on the action of synthetic pesticides that in some cases originate food pathogens that lead to severe foodbome diseases. There is a current need for new environmentally safe and efficient protective methods.

Microprotect project revisits this necessity by conceiving a methodology that exploits the antimicrobial and inseticide activity of essential oils, combined with a controlled release system, applied to cereal and bean storage.

Analysis of Internal Variables of a Distillation Column Operating with Distributed Control

P·FP6

G.N. Mellol

, R.A.F. Machadi, C. Marangonf" (1) University of Joinvllle Region, Joinville. Brazil, (2) Federal

University of Santa Catarina, Flonan6polis, Brazil (3) Federal University of Santa Catarina - Campus Blumenau.

Blumenau, Brazjl: 'cintia.ufscblumenau@gmail.com.

66

A proposal of a control system with distributed heating action between reboiler and one stage of stripping section has been studied, demonstrating reductions of the transition time when this strategy was compared to the conventional dual control. So, the objective of this work was to simulate the process with the distributed strategy analysing the internal profiles when feed

Ob . _ " , . . . _~_ temperature were disturbed. Tests were carried out using Aspen

1>1.",,,u .. d C~"!ro' Hysys® software and demonstrated that the internal profiles

show similar final steady states for temperature, pressure and ethanol fractions when distributed and conventional systems were used. An alteration in vapor flow inside the column due to the internal heat source was observed and this was reflected in reach faster the stationary state using the distributed approach. Poster session - Chemical Engineering Fundamentals and Practice ChennPor2014