Soluble prokaryotic overexpression and purification of bioactive human granulocyte colony-stimulating factor by maltose binding protein and protein disulfide isomerase.

Human intestinal epithelial cells (HIEC, lane 1 ), human intestinal mesenchymal cells (HIM, lane 2 ), 18-week fetal small intestine ( lane 3 ), and Caco-2 cells ( lane 4 )

lane 1, antigen with non- parasitized erythrocytes; lane 2, antigen of isolate from Southern region; lane 3, antigen of isolate from Southeastern region; lane 4, antigen of isolate

(a) Lane 1 , 10% SDS-PAGE profile of pooled human plasma after enrichment for normal VWF using ethanol precipitation; Lane 2 , western blotting for detection of normal VWF,

Lane M, DNA ladder; Lane 1, negative control non-transformed plants; Lane 2-11, independent transgenic lines; Lane 12, Positive control OsGSTL2 over-expression vector..

Lane 1, molecular mass standards (high mass of Invitrogen TM , silver stained); lane 2, crude extract; lane 3, thermally treated crude extract; lane 4, sample after

Lane 10: total protein of cells containing empty vector pET-28a with IPTG induction; Lane 11: total protein of cells containing expression vector pET- 28a-Ifu-chit2 without

Lane 1, manioc immunoblotting (noninhibited); Lane 2, inhibited with manioc extract (10 μL of manioc extract, concentration of 10 mg protein/mL) as positive control; Lane 3,

The recombinant pET-28a- hpaA was transformed into BL21 E.coli strains and the fusion protein was expressed... Lane1: Protein marker, Lane 2: Bacterial cell without plas- mid,