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Multiparametric Cell Cycle Analysis Using the Operetta High-Content Imager and Harmony Software with PhenoLOGIC.

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Academic year: 2017

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Fig 1. High-content cell cycle analysis based on DNA content. (A) Example images of HT29 cells with nuclei stained with Hoechst 33342 and then segmented with Harmony software using Find Nuclei Method B or Method M
Table 1. Agents used to validate image analysis methodology.
Fig 3. Validation of image analysis protocol. HT29 cells were treated with the indicated compound for 24 hour and then fixed and stained with Hoechst 33342, EdU and pHH3 (S10)
Fig 4. Determination of cell cycle phase of γH2AX positive cells following treatment with a cytotoxic DNA damaging agent
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