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Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: implications for calcification-related chronic inflammatory diseases

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Academic year: 2021

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Table 1. Gene-specific primers used in this study.
Fig 1. GRP and MGP are produced by leukocytes as γ-carboxylated proteins. Leukocyte subsets neutrophils (Neut), monocytes (Mon), and T lymphocytes (T-Lym) were isolated from human buffy coats, and macrophages (Mac) and dendritic cells (DC) were obtained by
Fig 2. γ-carboxylated GRP and MGP are produced in THP-1 cell line. THP-1 and THP-1 MoM differentiated with 25 ng/ml of PMA during 48h were cultured in control conditions and harvested for RNA and protein extraction.
Fig 3. GRP and MGP are up-regulated in LPS stimulated THP-1 cells. THP-1 and differentiated THP-1 macrophage cells were stimulated with 100 ng/
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