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Lipid modifications of Sonic hedgehog ligand dictate cellular reception and signal response.

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Academic year: 2017

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Figure 1. Schematics of experimentation. (A) Shh ligand lipidated with palmitate and cholesterol was generated from a full-length Shh open reading frame
Figure 2. Shh lipid modifications enhance cellular association.
Figure 3. Cholesterol is required for expression of Shh on the cell surface. Shown are flow cytometric data from NIH3T3 fibroblasts co- co-transfected with recombinant Shh variants (0.125 ng) and EGFP (1000 ng), and stained with 5E1-Alexa 647 conjugated an
Figure 4. Distinct properties of cholesterol and palmitate modification on cellular localization of Shh ligand
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