Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions.
Texto
Imagem
Documentos relacionados
Notably, when we subjected either rrf-1(pk1417) or rrf-1(ok589) mutants to elt-2 RNAi, we observed comparable phenotypes to those observed in wild-type animals grown on
We analyzed the status of the p53 network in various mutants for cell-essential genes. The genes affected in these mutants function in diverse cellular processes, yet their
Bacillus subtilis strain selection and construction of the RNAi vector Performing RNAi by feeding requires cloning a DNA fragment corresponding to the gene of interest into a vector
At the first stage of the measurements results analysis, the gear wheel cast surface image was compared with the casting mould 3D-CAD model (fig.. Next, the measurements results
We reduced the dose of the second RNAi ( lac2 RNAi) to 500 ng/mL to test whether we could make the assay system more specific to the genes involved in RNAi, however, the
As found with results from RNAi knockdown of EcR - USP (the ecdysone receptor genes), RNAi knockdown of Met at the early wandering stage disrupts the 20E-triggered
The advantages of using Drosophila cell lines for RNAi studies of mitosis include the sequenced and well annotated genome with a relatively high genetic conservation with humans
To address this, we took advantage of the tractable genetic model Caenorhabditis elegans expressing expanded polyglutamines (polyQ) and performed a genome-wide RNA interference