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Evaluation of cystamine-modified hyaluronic acid/chitosan polyplex as retinal gene vector

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Academic year: 2021

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Fig. 1. Schematic representation of modification reaction and conditions.
Diagram 2. Polyplex formulations with chitosan and hyaluronic acid. All polyplexes containing DNA were prepared at a N:P ratio of 15:1
Fig. 3. Size, polydispersity and zeta potential of polyplexes. Statistical differences and their significance are indicated by the star (*) symbol, with ***P b 0.001, ****P b 0.0001, ns — not significant.
Fig. 5. DNA complexation in polyplexes was analyzed by 1% agarose gel electrophoresis and DNA visualized with GreenSafe Premium after incubation with PBS or DMEM with 10% FBS for A) 3 and B) 7 days at 37 °C.
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