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Heterologous expression and biochemical characterization of an α1,2-mannosidase encoded by the Candida albicans MNS1 gene

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Academic year: 2019

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Fig.  1:  expression  of  Candida  albicans  Mns1   in  Escherichia  coli .  BL21  Star TM   (DE3)  cells  transformed  with  pMNS1c  were  grown  in  the absence or presence of 1 mM IPTG, and 500 μL from each culture  was removed at the times indicated
Fig. 2: A: purification of recombinant Mns1 by ion-exchange chromatography. The homogenate from cells expressing Candida albicans Mns1  was  applied onto a DEAE Bio-Gel A column (0.9 x 2.0 cm) equilibrated with buffer A
Fig. 4: immunodetection of the α1,2-mannosidases in the mixed mem- mem-brane and soluble fractions from Candida albicans
Fig. 5: analytical electrophoresis, zymogram analysis and immuno- immuno-blotting of partially purified membrane-bound α-mannosidase from  Candida  albicans;  Lane  1:  the  protein  profile  of  partially  purified  membrane-bound  enzyme  separated  by

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