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Meilan Xue, Yinlin Ge, Jinyu Zhang, Qing Wang and Lin Hou

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Academic year: 2019

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Figure 1.  Construction of the recombinant plasmid pEGFP-C3- pEGFP-C3-n-3. EGFP = enhanced green fluorescent protein; CMV = cyto  -megalovirus; FAD = fatty acid desaturase.
Figure 2.  Identification of the recombinant plasmids pEGFP-C3- pEGFP-C3-n-3  and  pEGFP-C3  by  double  enzyme  XhoI/EcoRI  digestion
Figure  4.  Result  of  n-3  fatty  acid  desaturase  (FAD)  transcript  levels  in  MCF-7  cells  transfected  with  pEGFP-C3-control  and  pEGFP-C3-n-3 after 48 h
Figure  5.  Partial gas chromatography traces showing fatty acid profiles of total cellular lipids extracted from non-transfected cells,  control cells transfected with control and cells transfected with n-3
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