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Differentiation Potential of O Bombay Human-Induced Pluripotent Stem Cells and Human Embryonic Stem Cells into Fetal Erythroid-Like Cells

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Academic year: 2017

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Fig 1: Schematic steps of erythroid differentiation by hiPSCs and hESCs. B-hiPS; Bombay human-induced pluripotent stem,  hES; Human embryonic stem, BMP; Bone morphogenetic protein, bFGF; Basic ibroblast growth factor, VEGF; Vascular  endothelial growth fac
Table 1: Real-time polymerase chain reaction (RT-PCR)  primers Annealing  tempera-ture (˚C)Primer sequenceGene 60F: 5´-CTCATTTCCTGGTATGACAACGA-3´ R: 5´-CTTCCTCTTGTGTTGCT-3´GAPDH 60F: 5´-TCGGCTGAGCTGAGAAATG-3´ R: 5´-GATGTCTTCGAGGTTCTCGG-3´RUNX1 60F: 5´-GAGG
Fig 2: Morphology, phenotype and mRNA analysis of B-hiPSC-derived cells before and after differentiation
Fig 3: Quantitative RT-PCR. Total mRNA of cells from indicated day were extracted and analyzed for expression of speciic  genes (table 1) by quantitative RT-PCR using the 2-∆∆CT method (n=3)
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