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Ancient origins: complement in invertebrates

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Academic year: 2017

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Fig. 1 Alignment of C3-like genes from the tunicate, H.  roretzi, (O97019) and the sea urchin S
Fig. 2 Western blots of C3-like proteins from the  tunicate, S. plicata. Proteins were immunoaffinity  purified with Cappel™ anti-human C3 and then  immunostained with either anti-human C3 from Sigma  Chemicals (F1, F2 and F3) or an antibody raised against
Fig. 3 Alignment of factor B genes from a variety of species. This region shows the highly conserved protease  signature sequence, [I/V]LTAAHCF, in red
Fig. 5 Alignment of four TEP sequences from D. melanogaster. These regions shows the highly conserved thiolester  motif in red and putative C3 α/β -like splice sites in blue

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