The second team of new players surprisingly includes subunits of proteasome parts that participate in antigen presentation to the immune system, i.e. The decision between apoptotic and antiapoptotic pathway induction is complex and multifactorial.
Transplantation of nP increased the number of proliferating cells in the neurogenic zones of the host brain. It was very important to observe the recovery of bioenergetic functions after mitochondrial transfer.
USE OF PHOTOCURING BIODEGRADABLE IMPLANTS BASED ON METHACRYLATED SILK FIBROIN FOR THE PREVENTION AND TREATMENT OF PATIENT INCORRECT. In the third group, 50% of the animals died from peritonitis as a result of intestinal anastomotic dehiscence.
OBTAining mixed culTureS Of neurOnS And gliA frOm humAn induced pluripOTenT
The developed protocol makes it possible to obtain neuronal / glial cell cultures in unlimited quantities using a bioreactor without expensive specific inducers and components of animal origin.
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The OBTAining Of criSpr/cAS9-mOdified humAn induced pluripOTenT STem cell line wiTh
To evaluate the therapeutic efficiency of the developed biocomposite hydrogel, we used the osteochondral defect model of rabbit joints. By changing the shape and length of the Tcm framework, we can use this technique to prepare tissue engineering constructs for the regeneration of both peripheral nerves and the damaged part of the spinal cord.
The mOdel Of BiOmedicAl cell prOducTS fOr preclinicAl STudieS
Scaffolds (certificate of priority not dated) are formed on the basis of cryoprecipitate of blood plasma obtained from healthy donors. The scaffolds formed on the basis of cryoprecipitate of pig blood plasma had the properties that did not differ from the scaffolds formed on the basis of cryoprecipitation of blood plasma from donors Subsequently, the formation of a dense cell network recorded in the same time period as in scaffolds based on blood plasma from healthy donors.
TO Be fiT fOr fAcTOry-TO-phArmAcy in The fuTure
The immediate task is to create cell lines, using the new infrastructure, in accordance with the requirements of the 180-fl and gmP recommendations and their banking. Pilot production of BmcP became part of the center of cell technologies, it is the competence center in regenerative medicine in Inc raS. The experience in the development of gmp manufacturing of biomedical cell products as a part of biomedical cell products as a part.
Of gxp quAliTy guidelineS in ruSSiA
It is achieved by introducing a multi-level system of control and quality assurance, regulation of all processes and automation or robotization of processes. The automatic cell processing system compact Select (TaP-Sartorius) enables us to robotize the basic stages of cell processing and is part of the technological chain. The work was carried out with the financial support of the project of the Russian Science Foundation.
SignificAnce Of pOly(c)-Binding prOTeinS fOr pluripOTenT STem cellS
The aim of this project was to identify new targets for immunotherapy in patients with soft tissue sarcomas. In the present work, the quantitative content of chemokine proteins in cultured cell supernatants of metastatic soft tissue sarcomas (STS) was evaluated, as well as the immunophenotype of peripheral blood Treg characterized by flow cytometry. Therefore, Tregs are a potential target for novel tumor therapy through inhibition of immunosuppression in the tumor site.
OBTAining inSulin-prOducing cellS frOm A nOnpAncreATic SOurce
The study was carried out within the state assignment of the Ministry of Health of the Russian Federation. Xenografting of human skin to the immunodeficient mice is one of the most valuable approaches for the in vivo investigation of human hair follicle morphogenesis. The shape and microstructure of the polymer substrate have a great effect on growth and differentiation of mScs, which places special requirements on the design of 3d scaffolds.
The study was carried out using biomaterials collected and preserved in the frame of rSf grant using the equipment purchased as part of lomonosov mSU development program and supported by rSf grant. In summary, the development of comprehensive and well-reasoned approaches for the standardization of cell-based regenerative medicine products that provide the reproducible pattern of cell heterogeneity is crucial for their effective translation into clinical practice. This study was carried out according to the Russian government program for competitive growth of Kazan Federal University and and through the subsidy awarded to Kazan Federal University for the state assignment in the field of scientific activities.
The aim of the work is thus to create a small diameter tissue engineering vessel (TeV) based on a polymeric scaffold populated by cells. The cell-free Pc vessel was sewn into the rat abdominal aorta, and blood flow was restored intraoperatively. The scaffold material did not cause any noticeable formation of microthrombi in the wall of the main artery lumen.
ShOrT TAndem repeAT prOfiling
Stem cell research has become one of the most studied areas in the life sciences because of its promising applications for tissue replacement therapy, disease modeling, and developmental biology. Oct4 has been shown to be a key regulator of stem cell pluripotency. The aim of the current research was to develop a new biomaterial based on decellularized ecm produced from human mSc cell sheets.
Of grAnulOSA frOm pOrcine fOllicleS
THE TOTAL METHYLATION LEVEL OF THE GENOME IN THE CELLS OF MOUSE TESTES AND ITS REGULATION IN THE CELLS OF MOUSE TESTES AND ITS REGULATION AFTER SIMULATED MICROGRAVITY. The aim of this study was to determine the level of total methylation and its regulation in the cells of the testes of the mice under antiorthostatic suspension for 30 days (group 30hS, n=7), followed by a 12-hour recovery (group 30hS+12h, n=7) and the corresponding control group c (n=7). The aim of the present studies was to evaluate the effect of induced overexpression of the klotho gene on the growth characteristics of tumor cells in vitro.
The aim of our research was morphological description of the non-healing wound model in mice. The h-shaped 10×30 mm skin flap and full thickness circular wound 5-7 mm in diameter in the center of the flap was applied to the back of an animal. The granulation tissue of a part of animals was less representative and was incorporated into subcutaneous tissue infiltrated by inflammatory cells.
The use of Tnf-alpha inhibitors in clinical practice is considered one of the greatest achievements of medicine in recent decades. Tnf-alpha-dependent expression of the cytokine interleukin-6 by human primary chondroblasts from different donors is shown. Thus, a fast, convenient and reliable in vitro method for screening compounds with Tnf-alpha inhibitory activity has been developed.
An increASe in numBer Of BlOOd veSSelS in The rAT regenerATing nerve AfTer
The purpose of the study of the functional state of mSc in contact with new anti-corrosion osteogenerative coatings deposited on magnesium and titanium. It was found that the most active cells adhered to the surface of the composite material Peo UdPfe coated on implants, while the number of these cells for magnesium and titanium without and with calcium phosphate Peo coated was significantly lower. The architecture of the mSc surface reflected the stimulating effect of the coatings, whereas no similar changes were observed on the surface of the implants.
Thus, when transplanted into the urothelial defect, autologous skin keratinocytes are incorporated into the structure of the urethra, restoring its integrity and functionality and acquiring phenotypic signs of urothelial cells. Thus, the tissue equivalent developed by us can be recommended as an alternative source of plastic material for urethral reconstruction with proximal forms of hypospadias.
The chondrocytes of the ear of mini pigs proliferate rapidly and are therefore a useful model to study glucose consumption by chondrocytes. The change in glucose concentration in the colorless medium dmem/f12 (gibco, USa) culture medium with 15% fBS (gibco, USa) in the growth dynamics of the culture of the minipig auricular chondrocytes was studied depending on the mode of culture medium change. It was shown that the active growth phase, regardless of the culture regimen, lasted two days in the culture of chondrocytes P0 and P1 passages.
Of primAry hippOcAmpAl culrureS IN VITRO
This is due to the genetically determined absence of vascular neoplasm in response to hypoxia in heart tissues. In view of the above, the aim of this study was to examine the possibility of correcting cirrhotic changes in the liver by administration of mmSc after transfection with their episomal vector containing hepatocyte growth factor (hgf). The indices of the IV group of animals did not differ from those of the control group.
Acknowledgments: low voltage scanning electron microscopy was performed using equipment of the Interdisciplinary Resource Center for Nanotechnology of St. Petersburg State University.
Relevance: In patients with cardiovascular pathology, endogenous c-kitpos cardiac stem cells (ecScs) are capable of generating cardiac muscle cells. Objective of the study: analysis of the ECSCs in patients of different ages with cardiovascular pathology. Research Methods: Biopsy specimens of the right ventricular (rV) myocardium from children with tetralogy of fallot (Tf; n months) and adult patients with hypertrophic cardiomyopathy (hcmP; n years) were studied.
The rOle Of “linKer” hmgB1 / hmgB2 prOTeinS in chrOmATin Of mOuSe STem cellS
Results: c-kitpos ecSc was found in the myocardium of children with Tf and adults with hcmP in a very low amount of 3-43 cells/106 cardiomyocytes. These small cells (diameter 5-7 µm) with a thin layer of cytoplasm containing sarcomeric α-actin were located in the interstitium. In the presented work, 3d multifunctional hydrogel cell carriers produced from modified high-molecular hyaluronic acid were designed, with the help of which a complex of tasks related to both biocompatibility and toxicity for nerve cells were solved.
The influence Of millimeTer wAve
Application of the tissue engineering graft for urethral reconstruction. Graft for urethral reconstruction. The main pathologies of the urethra are developmental abnormalities and acquired defects. Using antibodies against the Ser41 phosphorylated form of the protein, it was shown that gaP-43 is subject to phosphorylation by protein kinase c.