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Aqueous two phases systems

Extraction of biomolecules with aqueous two phases systems

Extraction of biomolecules with aqueous two phases systems

... alternatives for volatile and non-benign organic solvents in numerous fields [2, 3] . Indeed, these particular characteristics contributed to their classification as "green" solvents. This categorization yielded ...

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Thermoreversible (Ionic-Liquid-Based) Aqueous Biphasic Systems

Thermoreversible (Ionic-Liquid-Based) Aqueous Biphasic Systems

... the systems is cooled down to 25 °C the system becomes monophasic ...coexisting phases for a given initial ...liquid-liquid systems (UCST or LCST) 16,17,36–38 . Surfactant-based aqueous ...

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Liquid-liquid equilibria in aqueous two-phase ethanol/salt systems at different temperatures and their application to anthocyanins extraction

Liquid-liquid equilibria in aqueous two-phase ethanol/salt systems at different temperatures and their application to anthocyanins extraction

... Aqueous two-phase systems (ATPS) are formed by mixtures of chemical species that, under certain conditions, separate into two immiscible phases, with water as the major component in ...

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Use of aqueous two-phase PEG-salt systems for the removal of anionic surfactant from effluents.

Use of aqueous two-phase PEG-salt systems for the removal of anionic surfactant from effluents.

... For the experiments carried out at the bench scale, approxi- mately 400 g of salt and 300 g of polymer were weighed out (BL3200H, Martei, uncertainty of ±0.01 g) and mixed in a glass vat with a capacity of 3.0 L. About ...

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Are aqueous biphasic systems composed of deep eutectic solvents ternary or quaternary systems?

Are aqueous biphasic systems composed of deep eutectic solvents ternary or quaternary systems?

... used on DES preparation is not maintained in the coexisting phases of DES based ABS. This nonstoichiometric partitioning of the DES components also reinforces the hypothesis that these ABS are formed by the ...

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Development of purification platforms for immunoglobulin y using aqueous biphasic systems

Development of purification platforms for immunoglobulin y using aqueous biphasic systems

... mode the lightest phase is the mobile phase, flowing upwards through the heavier stationary phase, while the opposite occurs in the descending mode [8]. The main advantage of using CPC comes from the liquid-liquid nature ...

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Aqueous biphasic systems: a benign route using cholinium-based ionic liquids

Aqueous biphasic systems: a benign route using cholinium-based ionic liquids

... form two phases than ...its two hydroxyl terminal groups, no phase separation was in fact observed, as previously ...summary, two major factors were found to govern their salting-out/-in ...

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Extraction of antioxidants with aqueous biphasic systems

Extraction of antioxidants with aqueous biphasic systems

... of two immiscible aqueous phases has emerged as a more biocompatible, environmentally-friendly and economically feasible technique for the extraction/purification of several compounds ...when ...

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Partition study of textile dye Remazol Yellow Gold RNL in aqueous two-phase systems

Partition study of textile dye Remazol Yellow Gold RNL in aqueous two-phase systems

... This cation effect may be explained by the model of da Silva and Loh [30], which suggests that when mixing the polymer and salt, the cation of the salt and the macromolecules interact to release water molecules that were ...

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ARTIGO_New insights into the partitioning of phenothiazine dyes in aqueous two-phase systems

ARTIGO_New insights into the partitioning of phenothiazine dyes in aqueous two-phase systems

... parameter expresses the enthalpy change of the system when one mole of dye at infinitely diluted conditions is transferred from the bottom phase to the top phase of an ATPS. With this thermodynamic condition (infinite ...

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Recovery of pectinases from Aspergillus niger using aqueous two-phase systems / Recuperação de pectinases de Aspergillus niger usando sistemas aquosos bifásicos

Recovery of pectinases from Aspergillus niger using aqueous two-phase systems / Recuperação de pectinases de Aspergillus niger usando sistemas aquosos bifásicos

... technology, two-phase aqueous system (ATPS) prossesses attractive properties such as time saving, simplicity and easy scale-up that make its application economically feasible [3], technique that can be ...

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STUDY OF ALPHA-AMYLASE OBTAINED BY SOLID STATE FERMENTATION OF CASSAVA RESIDUE IN AQUEOUS TWO-PHASE SYSTEMS

STUDY OF ALPHA-AMYLASE OBTAINED BY SOLID STATE FERMENTATION OF CASSAVA RESIDUE IN AQUEOUS TWO-PHASE SYSTEMS

... using aqueous two-phase systems ...the aqueous two-phase system (ATPS) is the aqueous environment, which provides suitable conditions for the distribution of biomolecules, such ...

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Aqueous biphasic systems composed of ionic liquids and polysaccharides

Aqueous biphasic systems composed of ionic liquids and polysaccharides

... Before implementing their use in separation procedures, ABS must be fully characterized, namely each phase diagram (the binodal curve and the respective tie-lines (TLs)) that describes the system behavior for a given pH ...

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Enzymatic hydrolysis of sugarcane bagasse in aqueous two-phase systems (ATPS): exploration and conceptual process design

Enzymatic hydrolysis of sugarcane bagasse in aqueous two-phase systems (ATPS): exploration and conceptual process design

... by two immiscible components dissolved in water ( Benavides et ...presents two phases in which molecules (solutes) can be unevenly partitioned in accordance to the system composition ( Baskir et ...

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Progress in the Development of Aqueous Two-Phase Systems Comprising Ionic Liquids for the Downstream Processing of Protein- Based Biopharmaceuticals

Progress in the Development of Aqueous Two-Phase Systems Comprising Ionic Liquids for the Downstream Processing of Protein- Based Biopharmaceuticals

... polymer-salt systems as a way to increase the purification factors of a clinically relevant enzyme - ...respective phases, thereby allowing the reusing and recycling of both phases in new cycles of ...

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Purification of C-phycocyanin from Spirulina platensis in Aqueous Two-Phase Systems using an Experimental Design

Purification of C-phycocyanin from Spirulina platensis in Aqueous Two-Phase Systems using an Experimental Design

... the aqueous two-phase system (ATPS), a simple and economic system, has been ...of two polymers or of one polymer with a salt in a determined concentration, which assures the formation of two ...

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Enhanced performance of polymer-polymer aqueous two-phase systems using ionic liquids as adjuvants towards the purification of recombinant proteins

Enhanced performance of polymer-polymer aqueous two-phase systems using ionic liquids as adjuvants towards the purification of recombinant proteins

... separation. Phases (PEG-rich bottom and PPG-rich top phases) were isolated using a syringe and their weight ...Both phases and the dissolved precipitate were analyzed for total proteins and IFNα-2b ...

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An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems

An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems

... initial extraction steps all protein fractions are present in the system and each enzyme isoform is distributed between the phases, according to its structural properties. As the extraction process progresses ...

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Novel aqueous micellar two-phase systems with ionic liquids

Novel aqueous micellar two-phase systems with ionic liquids

... (28–31). These techniques have been applied in several types of compounds and processes, for example in the removal of metal ions by LLE (32–34) and LPME (35); in the extraction of phenolic biomolecules (36) accomplished ...

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Purification of antibodies using aqueous two-phase systems

Purification of antibodies using aqueous two-phase systems

... potential systems capable of selectively extracting IgY from egg yolk, the total protein partition coefficients in each ATPS and the respective proteins profile were also ...and phases’ ...

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